Anti-hyperlipi-demic effects of polysaccharide MA from Mytilus coruscus on experimental atherosclerosis in rabbits
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摘要: 目的 探讨贻贝多糖MA对实验性动脉粥样硬化兔的降血脂作用及其机制,并观察其对肝脏和主动脉的影响。 方法 采用喂养高脂饲料的方法建立实验性动脉粥样硬化兔模型,连续给予MA 4周后,测定兔血脂水平,如血清三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)、高密度脂蛋白胆固醇(HDL)水平,以及与血脂代谢相关酶如脂蛋白酯酶(LPL)、肝酯酶(HL)、羟甲基戊二酸单酰辅酶-A还原酶(HMG-CoAR)活性;并进行肝脏的病理组织学和超微结构检查;油红染色观察兔主动脉粥样硬化斑块的脂质沉积情况。 结果 贻贝多糖MA能显著降低实验性动脉粥样硬化兔血清TG、TC、LDL水平,并显著增加HL和 LPL的活性(P<0.05)。MA能减轻肝脏脂质沉积,减少主动脉粥样硬化斑块的脂质沉积和斑块形成。 结论 贻贝多糖MA通过增加HL和 LPL活性发挥调血脂功能,可用于防治家兔动脉粥样硬化和脂肪肝。Abstract: Objective To explore the anti-hyperlipidemic effects and mechanism of polysaccharide MA from Mytilus coruscus in the experimental atherosclerosis rabbits and to observe the effects of MA on liver and aorta. Methods The atherosclerosis rabbit model was established by high-fat diet. The blood lipid such as triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL) in serum and enzymes metabolizing blood lipid such as lipoprteinlipase (LPL), hepaticlipase (HL) and hydroxymethylglutaryl CoA reductase (HMG-CoAR) were measured after atherosclerosis rabbits fed MA for 4 weeks. Histopathology and ultramicroscopic structure of hepatic tissue was observed. Lipid storage in aortic atherosclerotic plaque was observed by the oil red staining method. Results MA significantly reduced the level of TG, TC, LDL in serum and increased HL, LPL (P<0.05). MA reduced lipid storage in liver and aortic atherosclerotic plaque. Conclusion MA might play regulating effects in serum lipid by enhancing the activities of LPL and HL, and inhibit formation of atherosclerosis and fatty liver.
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Key words:
- atherosclerosis /
- Mytilus coruscus /
- polysaccharide /
- blood lipid
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[1] 姚 颖,魏江州,汪 俊,等.厚壳贻贝多糖的提取和免疫学活性研究[J].第二军医大学学报, 2005, 26:896-899. [2] 徐红丽,郭婷婷,郭一峰,等.贻贝水溶性多糖MP-I的分离及体外抗肿瘤活性[J].第二军医大学学报,2006, 27:998-1001. [3] Jung W K, Qian Z J, Lee Z J, et al. Free radical scavenginh activity of a novel antioxidative peptide isolated from in vitro gastrointestinal digests of mytilus coruscus[J]. J Med Food,2007, 10:197-202. [4] 刘志峰,李 萍.贻贝提取物抗高血脂作用的观察[J].中国海洋药物,2001,20:9-10. [5] 明 亮,邵伯芹,张 艳,等.贻贝多活素对鹌鹑实验性动脉粥样硬化的影响[J]. 中国海洋药物,1996,12(6):554-557. [6] 储智勇,毛俊琴,李铁军,等. 贻贝多糖对大鼠高脂血症的影响[J]. 解放军药学学报,2008,24(3):213-215. [7] 马明华,易杨华,汤海峰.厚壳贻贝多糖MA的分离纯化、理化性质及活性研究[J].中国海洋药物,2004, 4:14-18. [8] 李 玲,储智勇,袁 波,等.贻贝多糖胶囊辅助降血脂人体试食研究[J].中药材,2011,34(10):1645-1647. [9] 吴晓霞,李建科,刘迎利.蚕蛹油α-亚麻酸对实验性高血脂大鼠的降脂作用[J]. 食品科学,2011,32(15):285-288. [10] 于竹芹,曾 锋,徐新颖.羊栖菜对实验性高脂血症大鼠血脂水平的调节作用[J]. 中国海洋药物杂志,2011,30(1):45-48. [11] 吴 涓,孙 倩,储智勇.贻贝多糖胶囊毒理学安全性研究[J]. 中国卫生检验杂志,2011,21(1):97-100. -
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