SHANG Liuwenxin, SUN Xin, PENG Cheng, XIE Hehui, CHEN Fengyuan, ZHANG Chuan. Improvement of impaired endothelial progenitor cell functions by in vitro drug interference in DOCA-salt hypertensive mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(3): 221-226. doi: 10.12206/j.issn.1006-0111.201912074
Citation:
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SHANG Liuwenxin, SUN Xin, PENG Cheng, XIE Hehui, CHEN Fengyuan, ZHANG Chuan. Improvement of impaired endothelial progenitor cell functions by in vitro drug interference in DOCA-salt hypertensive mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(3): 221-226. doi: 10.12206/j.issn.1006-0111.201912074
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Improvement of impaired endothelial progenitor cell functions by in vitro drug interference in DOCA-salt hypertensive mice
- 1.
Department of Authentication of Chinese Medicines, Naval Medical University, Shanghai 200433, China
- 2.
School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
- Received Date: 2019-12-16
- Rev Recd Date:
2020-03-05
Available Online:
2020-05-20
- Publish Date:
2020-05-01
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Abstract
Objective To investigate the effect of tetrahydrobiopterin (BH4), superoxide dismutase-polyethylene glycol (PEG-SOD) and N(G)-nitro-L-arginine (L-NNA) on impaired endothelial progenitor cell (EPC) functions in DOCA-salt hypertensive mice. Methods DOCA-salt hypertension was created and systolic blood pressure was measured by tail-cuff methods. EPC was isolated from bone marrow of mice and characterized by flow cytometry and fluorescence microscopy. EPC of DOCA-salt mice was incubated with BH4, PEG-SOD, and L-NNA for 24 h, then in vitro EPC function assays were performed. Results Compared with control group, systolic blood pressure was significantly increased in DOCA-salt mice. Both EPC adhesion and angiogenesis functions were impaired in DOCA-salt mice compared to control animals, which were reversed by incubation with BH4, PEG-SOD and L-NNA. Conclusion BH4, PEG-SOD and L-NNA rescued the impairments from EPC functions in DOCA-salt hypertensive mice.
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Proportional views
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