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Volume 30 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(1): 29-31

SONG Xiao-nan, WANG Xiao-bo, XI Rong-gang, MA Wen-hui, YAN Dong. Determination of ambroxol hydrochloride in humam plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 29-31. doi: 10.3969/j.issn.1006-0111.2012.01.007
Citation: SONG Xiao-nan, WANG Xiao-bo, XI Rong-gang, MA Wen-hui, YAN Dong. Determination of ambroxol hydrochloride in humam plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 29-31. doi: 10.3969/j.issn.1006-0111.2012.01.007
shu

Determination of ambroxol hydrochloride in humam plasma by HPLC

doi: 10.3969/j.issn.1006-0111.2012.01.007
  • 1.

    No. 210 Hospital of PLA, Dalian 116013,China

  • 2.

    Shanghai Hengshan Medicine Industry Co.Ltd,Shanghai 200233,China

  • 3.

    E-LIVING Pharmaceutical Co.Ltd,Shenyang 110179,China

  • Received Date: 2011-10-26
  • Rev Recd Date: 2011-12-25
  • Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride. Methods A Diamonsil-C18(200 mm×4.6 mm,5μm)column was used.The mobile phase consisted of methanol-0.01 mol/L PBS(pH 7.0)-THF(700:320:25,v/v/v).Detection wavelength was 242 nm. Results A good linear relationship was obtained in the concentration range of 10.2~610.8 ng/ml. The average recovery of this method was 88.2％~104.8％. The RSD of within-day and between-day were all less than 15％. Conclusion The method was simple,rapid and accurate. The result showed that the method was suitable for the determination of ambroxol hydrochloride.
  • [1] 国家药典委员会. 中华人民共和国药典临床用药须知:化学药和生物制品卷[M]. 北京:人民卫生出版社,2005:242.
    [2] NobilisM,Pastera J,SvobodaD,et al.High-performance liquid chromatography determination of ambroxol in human plasma[J].J Chromatogr,1992,581:251.
    [3] 钟大放. 以加权最小二乘法建立生物分析标准曲线的若干问题[J] . 药物分析杂志,1996,16(5):343.
    [4] 戈升荣,安富荣,曹惠明,等. 高效液相色谱法测定人血浆中盐酸氨溴索浓度[J]. 中国药房,2004,15(1):37.
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  • 1. 

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Determination of ambroxol hydrochloride in humam plasma by HPLC

doi: 10.3969/j.issn.1006-0111.2012.01.007
  • 1. No. 210 Hospital of PLA, Dalian 116013,China
  • 2. Shanghai Hengshan Medicine Industry Co.Ltd,Shanghai 200233,China
  • 3. E-LIVING Pharmaceutical Co.Ltd,Shenyang 110179,China
  • Received Date: 2011-10-26
  • Rev Recd Date: 2011-12-25

Abstract: Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride. Methods A Diamonsil-C18(200 mm×4.6 mm,5μm)column was used.The mobile phase consisted of methanol-0.01 mol/L PBS(pH 7.0)-THF(700:320:25,v/v/v).Detection wavelength was 242 nm. Results A good linear relationship was obtained in the concentration range of 10.2~610.8 ng/ml. The average recovery of this method was 88.2％~104.8％. The RSD of within-day and between-day were all less than 15％. Conclusion The method was simple,rapid and accurate. The result showed that the method was suitable for the determination of ambroxol hydrochloride.

SONG Xiao-nan, WANG Xiao-bo, XI Rong-gang, MA Wen-hui, YAN Dong. Determination of ambroxol hydrochloride in humam plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 29-31. doi: 10.3969/j.issn.1006-0111.2012.01.007
Citation: SONG Xiao-nan, WANG Xiao-bo, XI Rong-gang, MA Wen-hui, YAN Dong. Determination of ambroxol hydrochloride in humam plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 29-31. doi: 10.3969/j.issn.1006-0111.2012.01.007
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