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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(1): 42-44,48

DOU Ying-Hui, WANG Li, HE Guang-Wei, WU Qiang, FAN Guo-Rong, HANG Tai-Jun. Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 42-44,48. doi: 10.3969/j.issn.1006-0111.2012.01.011
Citation: DOU Ying-Hui, WANG Li, HE Guang-Wei, WU Qiang, FAN Guo-Rong, HANG Tai-Jun. Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 42-44,48. doi: 10.3969/j.issn.1006-0111.2012.01.011
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Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2012.01.011
  • 1.

    Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;School of Pharmacy, Second Military Medical University, Shanghai, 200433, China

  • 2.

    Hefei Medical Engineering Pharmaceutical Co., Ltd., Hefei 230088, China

  • 3.

    School of Pharmacy, Second Military Medical University, Shanghai, 200433, China

  • 4.

    Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China

  • Received Date: 2011-04-08
  • Rev Recd Date: 2011-11-06
  • Objective To establish a method for determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in plasma of SD rats by RP-HPLC. Methods The chromatographic separation was performed on a Diamonsil-C18 column(100 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile/5 mM ammonium acetate buffer(pH 4.0)(30/70,v/v). The single intravenous injection of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate was given to 70 SD rats to study the pharmacokinetics. Results The intra-run and inter-run RSD were less than 15%. The pharmacokinetics parameters were as follows: Cmax was 35.60 μg/ml , t1/2 was 3.36 h, AUC0~12 was 17.12 μg h/ml, AUC0~∞ was 17.72μg h/ml. Conclusion The method was simple and accurate, which could be used to separation and determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate.
  • [1] 潘赞红, 金 鑫. 川芎嗪与阿魏酸配伍对大鼠心肌缺血再灌注损伤心肌的保护作用[J]. 天津医药. 2008, 36(10): 796.
    [2] 杨家荣, 张密霞, 常亮堂, 等. 川芎嗪、阿魏酸及其配伍对心肌缺血再灌注模型大鼠的保护作用及对黏附分子的影响[J]. 中草药. 2008, 39(7): 1054.
    [3] 杜 希. 氯吡格雷的药理作用与临床评价[J]. 首都医药. 2002, 7: 44.
    [4] 李 琳, 张临洪, 徐武平. 氯吡格雷联合奥扎格雷钠治疗后循环脑梗死的临床疗效观察[J].中国临床神经外科杂志. 2010, 15(8): 474.
    [5] 王茜莎, 潘雪刁, 张德志, 等. 奥扎格雷钠对心肌缺血的保护作用研究[J]. 广东药学院学报. 2010, 26(3): 292.
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Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2012.01.011
  • 1. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;School of Pharmacy, Second Military Medical University, Shanghai, 200433, China
  • 2. Hefei Medical Engineering Pharmaceutical Co., Ltd., Hefei 230088, China
  • 3. School of Pharmacy, Second Military Medical University, Shanghai, 200433, China
  • 4. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China
  • Received Date: 2011-04-08
  • Rev Recd Date: 2011-11-06

Abstract: Objective To establish a method for determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in plasma of SD rats by RP-HPLC. Methods The chromatographic separation was performed on a Diamonsil-C18 column(100 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile/5 mM ammonium acetate buffer(pH 4.0)(30/70,v/v). The single intravenous injection of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate was given to 70 SD rats to study the pharmacokinetics. Results The intra-run and inter-run RSD were less than 15%. The pharmacokinetics parameters were as follows: Cmax was 35.60 μg/ml , t1/2 was 3.36 h, AUC0~12 was 17.12 μg h/ml, AUC0~∞ was 17.72μg h/ml. Conclusion The method was simple and accurate, which could be used to separation and determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate.

DOU Ying-Hui, WANG Li, HE Guang-Wei, WU Qiang, FAN Guo-Rong, HANG Tai-Jun. Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 42-44,48. doi: 10.3969/j.issn.1006-0111.2012.01.011
Citation: DOU Ying-Hui, WANG Li, HE Guang-Wei, WU Qiang, FAN Guo-Rong, HANG Tai-Jun. Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 42-44,48. doi: 10.3969/j.issn.1006-0111.2012.01.011
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