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WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
Citation:
DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
Determination of polydatin in rats plasma by LC-MS/MS
Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
Received Date: 2011-02-10
Rev Recd Date:
2011-12-21
Abstract
Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C18 reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
Gao S, Fan G, Hong Z, et al. HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies[J]. J Pharmaceut Biomed,2006, 41(1): 240.
Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C18 reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
Citation:
DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012
Gao S, Fan G, Hong Z, et al. HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies[J]. J Pharmaceut Biomed,2006, 41(1): 240.
Zhou S, Yang R,Teng Z,et al. Dose-dependent absorption and metabolism of trans-polydatin in rats[J]. J Agr Food Chem, 2009,57(11): 4572.
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WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
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