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WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
Citation:
WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
Determination of sodium Danshensu in rat liver by HPLC-MS/MS
New drug research center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Deportment of pharmacy,Qingpu Distric Central Hosptal,Shanghai 201700, China
2.
Center of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3.
New drug research center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Received Date: 2011-04-21
Rev Recd Date:
2011-06-14
Abstract
Objective To establish a HPLC-MS/MS method for determination of sodium Danshensu in SD rat liver. Methods Tissue samples were pretreated by liquid-liquid extraction method after homogenate, and ketoprofen was used as the internal standard. Analytical column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase was methanol: water(0.01‰ formic acid)=80:20 with flow rate as 1.0 ml/min. Mass spectrum determination was performed in the MRM mode with target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen), using ESI ion resource and negative ion detection. Results The calibration curve was linear over the range of 20.2~1 010 ng/ml. The LLOQ of sodium Danshensu in liver was 20.2 ng/ml. The intra-and inter-day RSD were both less than 10%. The extracted recovery rate ranged from 92% to 107%. Conclusion The method was sensitive, rapid, simple and accurate to determine sodium Danshensu distribution in rat liver and was suitable for preclinical test of Sodium Danshensu.
Lain FFY,Yeung JHK,Chan KM,et al.Relaxant effects of danshen aqueous extract and its constituent danshensu on rat coronary artery are mediated by inhibition of calcium channels[J]. Vascular Pharmacology, 2007, 46: 271.
[2]
Huang YS, Zhang JT. Antioxidative effect of three watersoluble components isolated from Salvia miltiorrhiza in vitro[J]. Acta Pharm Sin, 1992, 27(2): 96.
Sun XM, Cai HJ, Song QY, et al. Studies on new pharmacological action of an extract from Danshen (Salvia miltiorrhiza)[J]. Chin Tradit Herb Drugs, 1991, 22(1): 20.
[5]
Zhang LJ, Chen L, Lu Y, et al. Danshensu has anti-tumor activity in B16F10 melanoma by inhibiting angiogenesis and tumor cell invasion[J]. Int J Pharm, 2010, 643(2-3): 195.
[6]
Zhao GR, Zhang HM, Ye TX, et al. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B[J]. Food ChemToxicol, 2008, 46(1): 73.
Li XL, Li XR. Simultaneous determination of Danshensu, ferulic acid, cryptotanshinone and tanshinone IIA in rabbit plasma by HPLC and their pharmacokinetic application in danxiongfang[J]. J Pharmaceut Biomed, 2007, 44(5): 1106.
1. New drug research center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Deportment of pharmacy,Qingpu Distric Central Hosptal,Shanghai 201700, China
2. Center of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3. New drug research center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Abstract: Objective To establish a HPLC-MS/MS method for determination of sodium Danshensu in SD rat liver. Methods Tissue samples were pretreated by liquid-liquid extraction method after homogenate, and ketoprofen was used as the internal standard. Analytical column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase was methanol: water(0.01‰ formic acid)=80:20 with flow rate as 1.0 ml/min. Mass spectrum determination was performed in the MRM mode with target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen), using ESI ion resource and negative ion detection. Results The calibration curve was linear over the range of 20.2~1 010 ng/ml. The LLOQ of sodium Danshensu in liver was 20.2 ng/ml. The intra-and inter-day RSD were both less than 10%. The extracted recovery rate ranged from 92% to 107%. Conclusion The method was sensitive, rapid, simple and accurate to determine sodium Danshensu distribution in rat liver and was suitable for preclinical test of Sodium Danshensu.
WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
Citation:
WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
Lain FFY,Yeung JHK,Chan KM,et al.Relaxant effects of danshen aqueous extract and its constituent danshensu on rat coronary artery are mediated by inhibition of calcium channels[J]. Vascular Pharmacology, 2007, 46: 271.
[2]
Huang YS, Zhang JT. Antioxidative effect of three watersoluble components isolated from Salvia miltiorrhiza in vitro[J]. Acta Pharm Sin, 1992, 27(2): 96.
Sun XM, Cai HJ, Song QY, et al. Studies on new pharmacological action of an extract from Danshen (Salvia miltiorrhiza)[J]. Chin Tradit Herb Drugs, 1991, 22(1): 20.
[5]
Zhang LJ, Chen L, Lu Y, et al. Danshensu has anti-tumor activity in B16F10 melanoma by inhibiting angiogenesis and tumor cell invasion[J]. Int J Pharm, 2010, 643(2-3): 195.
[6]
Zhao GR, Zhang HM, Ye TX, et al. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B[J]. Food ChemToxicol, 2008, 46(1): 73.
Li XL, Li XR. Simultaneous determination of Danshensu, ferulic acid, cryptotanshinone and tanshinone IIA in rabbit plasma by HPLC and their pharmacokinetic application in danxiongfang[J]. J Pharmaceut Biomed, 2007, 44(5): 1106.
WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
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