Pharmacology studies on acute toxicity and safety of imperatorin

ZHU Hai; SUN Yi; XIA Zhen-na; GAO Ting-ting; DU Chun-hui; LU Wen; GONG Xue-lian

doi:10.3969/j.issn.1006-0111.2013.06.008

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To determine the acute toxicity of imperatorin on rats and mice, and the influence of imperatorin on the nervous system of mice and the cardiovascular and respiratory systems of beagle dogs. Methods intraperitoneal injection (ip) and intragastric administration (ig) were performed to mice and ig to rats to determine the acute toxicity of imperatorin. In safety pharmacology experiments, ip of imperatorin was performed to mice in single doses of 50, 100, and 200 mg/kg to observe its influence on the nervous system of mice (including locomotor activity, pole climbing ability, and collaborative hyposis experiment of threshold dose of sodium pentobarbital, etc.). Oral administration (po) of imperatorin was also performed in single doses of 25, 50, 100 mg/kg to beagle dogs to observe its influence on the cardiovascular and respiratory systems of dogs (including ECG, blood pressure, heart rate, and breathing indexes, etc.). Results The LD₅₀ of imperatorin to mice is 988.5 mg/kg by ig and 603.3 mg/kg by ip, and to rats is 3188.7 mg/kg by ig. No significant effects on the nervous system of mice were observed by single ip of imperatorin. And no significant effects on the cardiovascular and respiratory systems of beagle dogs were observed by single po of imperatorin. Conclusion Imperatorin had a wide range of safety application, and its toxicity was relatively easy to control. The antihypertensive effects of imperatorin on normal animals (including mice, rats and dogs) were not significant, therefore it had a great possibility in clinical application.

Pharmacology studies on acute toxicity and safety of imperatorin

1. Department of Hygiene and Health Toxicology, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China

2. Reseearch & Engineering Center for Natural Medicine, Xi'an Jiaotong University, Xi'an 710061, China

Received Date: 2012-12-19

Rev Recd Date: 2013-04-08

Key words:

Abstract: Objective To determine the acute toxicity of imperatorin on rats and mice, and the influence of imperatorin on the nervous system of mice and the cardiovascular and respiratory systems of beagle dogs. Methods intraperitoneal injection (ip) and intragastric administration (ig) were performed to mice and ig to rats to determine the acute toxicity of imperatorin. In safety pharmacology experiments, ip of imperatorin was performed to mice in single doses of 50, 100, and 200 mg/kg to observe its influence on the nervous system of mice (including locomotor activity, pole climbing ability, and collaborative hyposis experiment of threshold dose of sodium pentobarbital, etc.). Oral administration (po) of imperatorin was also performed in single doses of 25, 50, 100 mg/kg to beagle dogs to observe its influence on the cardiovascular and respiratory systems of dogs (including ECG, blood pressure, heart rate, and breathing indexes, etc.). Results The LD₅₀ of imperatorin to mice is 988.5 mg/kg by ig and 603.3 mg/kg by ip, and to rats is 3188.7 mg/kg by ig. No significant effects on the nervous system of mice were observed by single ip of imperatorin. And no significant effects on the cardiovascular and respiratory systems of beagle dogs were observed by single po of imperatorin. Conclusion Imperatorin had a wide range of safety application, and its toxicity was relatively easy to control. The antihypertensive effects of imperatorin on normal animals (including mice, rats and dogs) were not significant, therefore it had a great possibility in clinical application.

HTML

Reference (13)

[1]	张利,马逾英,蒋桂华,等.白芷新型饮片中总香豆素与欧前胡素的含量测定[J].时珍国医国药,2011,22(1):80.
[2]	刘笑笑,曹蔚,王四旺.欧前胡素的提取分离方法和药理学研究进展[J].现代生物医学进展,2010,20(10):3954.
[3]	何开家,张涵庆.白芷化学成分及其药理研究进展[J].现代中药研究与实践,2008,22(3):59.
[4]	Aída N. García-Argáez,Teresa O. Ramírez Apan,Hortensia Parra Delgado,et al.Anti-inflammatory activity of coumarins from decatropis bicoloron TPA ear mice model[J].Planta Med,2000,66(3):279.
[5]	Kawaii S,Tomono Y,Ogawa K,et al.Antiproliferative effect of isopentenylated coumarins on several cancer cell lines[J].Anticancer Res,2001,21(3B):1905.
[6]	Sergio Rosselli,Antonella Maggio,Gabriella Bellone,et al.Antibacterial and anticoagulant activities of coumarins isolated from the flowers of magydaris tomentosa[J].Planta Med,2007,73(2):116.
[7]	He JY,Zhang W,He LC,et al.Imperatorin induces vasodilatation possibly via inhibiting voltage dependent calcium channel and receptor-mediated Ca²⁺ influx and release[J].Eur J Pharmacol,2007,573(1-3):170.
[8]	Zhang Y,Cao YJ,Zhan YZ,et al.Furanocoumarins-imperatorin inhibits myocardial hypertrophy both in vitro and in vivo[J].Fitoterapia,2010,81(8):1188.
[9]	Zhang Y,Cao YJ,Duan HJ,et al.Imperatorin prevents cardiac hypertrophy and the transition to heart failure via NO-dependent mechanisms in mice[J].Fitoterapia,2012,83(1):60.
[10]	郝光荣.实验动物学[M].2版.上海:第二军医大学出版社,2002:183.
[11]	袁伯俊,廖明阳,李波.药物毒理学实验方法与技术[M].北京:化学工业出版社,2007:140.
[12]	陆国才,袁伯俊.新药研究与评价[M].上海:第二军医大学出版社,2011:78.
[13]	徐叔云,卞如濂,陈修.药理实验方法学[M].2版.北京:人民卫生出版社,1994:657.

Message Board

Pharmacology studies on acute toxicity and safety of imperatorin

doi: 10.3969/j.issn.1006-0111.2013.06.008

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views