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| Citation:
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JI Song-gang, YANG Yu, LI Kang, WU Hai-tang, ZHU Zhen-yu. Determination of polyamines in Candida albicans by Pre-column derivation-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 412-414,453. doi: 10.3969/j.issn.1006-0111.2013.06.04
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Determination of polyamines in Candida albicans by Pre-column derivation-HPLC
- 1.
Department of Pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China
- 2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 3.
No. 169 Hospital of PLA, Hengyang 421000, China
- Received Date: 2013-08-28
- Rev Recd Date:
2013-10-31
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Abstract
Objective To develop a HPLC method for simultaneous determination of four polyamines (putrescine, cadaverine, spermidine, spermine) in Candida albicans. Methods HPLC analysis was performed on a Shiseido C18 column(100 mm×3.0 mm, 3.0 μm) after benzoyl chloride derivatization.The mobile phase consist of methanol (A) and water containing 0.1%methane acid (B). The gradient elution program was as follow:0~5 min, 30%~48% B; 5~12 min, 48% B;12~15 min, 48%~70% B;15~25 min, 70% B, the flow rate was 0.6 ml/min, the detection wavelength was 254 nm. Results The linearity was obtained over 0.775~77.50 μg/ml (r=0.999 9) for putrescine, 1.160~116.0 μg/ml (r=0.999 9) for cadaverine, 5.800~580.0 μg/ml (r=0.999 8) for spermidine and 2.533~192.7 μg/ml (r=0.999 9) for spermine. The average recoveries were between 94.27%~109.3%.The RSDs of precision were less than 4%. Conclusion The present study provided a sensitive and reproducible method for the simultaneously determination of four polyamines levels in Candida albicans.
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