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YANG Jiao, CHEN Junjun, ZHANG Xiangqi, ZHANG Jingxian, HAN Yonglong. Antitumor activity of Xiaoaiping injection combined with paclitaxel on ovarian cancer SK-OV-3 cells and nude mouse with ovarian cancer SK-OV-3 transplantation tumor[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 516-519,529. doi: 10.3969/j.issn.1006-0111.2017.06.009
Citation:
JI Song-gang, YANG Yu, LI Kang, WU Hai-tang, ZHU Zhen-yu. Determination of polyamines in Candida albicans by Pre-column derivation-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 412-414,453. doi: 10.3969/j.issn.1006-0111.2013.06.04
Determination of polyamines in Candida albicans by Pre-column derivation-HPLC
Department of Pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China
2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3.
No. 169 Hospital of PLA, Hengyang 421000, China
Received Date: 2013-08-28
Rev Recd Date:
2013-10-31
Abstract
Objective To develop a HPLC method for simultaneous determination of four polyamines (putrescine, cadaverine, spermidine, spermine) in Candida albicans. Methods HPLC analysis was performed on a Shiseido C18 column(100 mm×3.0 mm, 3.0 μm) after benzoyl chloride derivatization.The mobile phase consist of methanol (A) and water containing 0.1%methane acid (B). The gradient elution program was as follow:0~5 min, 30%~48% B; 5~12 min, 48% B;12~15 min, 48%~70% B;15~25 min, 70% B, the flow rate was 0.6 ml/min, the detection wavelength was 254 nm. Results The linearity was obtained over 0.775~77.50 μg/ml (r=0.999 9) for putrescine, 1.160~116.0 μg/ml (r=0.999 9) for cadaverine, 5.800~580.0 μg/ml (r=0.999 8) for spermidine and 2.533~192.7 μg/ml (r=0.999 9) for spermine. The average recoveries were between 94.27%~109.3%.The RSDs of precision were less than 4%. Conclusion The present study provided a sensitive and reproducible method for the simultaneously determination of four polyamines levels in Candida albicans.
Abstract: Objective To develop a HPLC method for simultaneous determination of four polyamines (putrescine, cadaverine, spermidine, spermine) in Candida albicans. Methods HPLC analysis was performed on a Shiseido C18 column(100 mm×3.0 mm, 3.0 μm) after benzoyl chloride derivatization.The mobile phase consist of methanol (A) and water containing 0.1%methane acid (B). The gradient elution program was as follow:0~5 min, 30%~48% B; 5~12 min, 48% B;12~15 min, 48%~70% B;15~25 min, 70% B, the flow rate was 0.6 ml/min, the detection wavelength was 254 nm. Results The linearity was obtained over 0.775~77.50 μg/ml (r=0.999 9) for putrescine, 1.160~116.0 μg/ml (r=0.999 9) for cadaverine, 5.800~580.0 μg/ml (r=0.999 8) for spermidine and 2.533~192.7 μg/ml (r=0.999 9) for spermine. The average recoveries were between 94.27%~109.3%.The RSDs of precision were less than 4%. Conclusion The present study provided a sensitive and reproducible method for the simultaneously determination of four polyamines levels in Candida albicans.
YANG Jiao, CHEN Junjun, ZHANG Xiangqi, ZHANG Jingxian, HAN Yonglong. Antitumor activity of Xiaoaiping injection combined with paclitaxel on ovarian cancer SK-OV-3 cells and nude mouse with ovarian cancer SK-OV-3 transplantation tumor[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 516-519,529. doi: 10.3969/j.issn.1006-0111.2017.06.009
Citation:
JI Song-gang, YANG Yu, LI Kang, WU Hai-tang, ZHU Zhen-yu. Determination of polyamines in Candida albicans by Pre-column derivation-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 412-414,453. doi: 10.3969/j.issn.1006-0111.2013.06.04
Cao YY, Zhu ZZ,Chen XF,et al. Effect of amphotericin B on the metabolic profiles of Candida albicans[J]. J Proteome Res, 2013,12(6):2921.
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YANG Jiao, CHEN Junjun, ZHANG Xiangqi, ZHANG Jingxian, HAN Yonglong. Antitumor activity of Xiaoaiping injection combined with paclitaxel on ovarian cancer SK-OV-3 cells and nude mouse with ovarian cancer SK-OV-3 transplantation tumor[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 516-519,529. doi: 10.3969/j.issn.1006-0111.2017.06.009
YANG Jiao, CHEN Junjun, ZHANG Xiangqi, ZHANG Jingxian, HAN Yonglong. Antitumor activity of Xiaoaiping injection combined with paclitaxel on ovarian cancer SK-OV-3 cells and nude mouse with ovarian cancer SK-OV-3 transplantation tumor[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 516-519,529. doi: 10.3969/j.issn.1006-0111.2017.06.009
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