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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2014  >  32(1): 35-41,48

Tan Hexin, Liu Ying, Zhang Lei. Cloning and molecular characterization of fructose-1, 6-bisphosphate aldolase gene from Salvia miltiorrhiza[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 35-41,48. doi: 10.3969/j.issn.1006-0111.2014.01.009
Citation: Tan Hexin, Liu Ying, Zhang Lei. Cloning and molecular characterization of fructose-1, 6-bisphosphate aldolase gene from Salvia miltiorrhiza[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 35-41,48. doi: 10.3969/j.issn.1006-0111.2014.01.009
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Cloning and molecular characterization of fructose-1, 6-bisphosphate aldolase gene from Salvia miltiorrhiza

doi: 10.3969/j.issn.1006-0111.2014.01.009

  • Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2012-12-19
  • Rev Recd Date: 2013-10-29
  • Salvia miltiorrhiza (Bung), a famous medicinal plant, is widely used in China, Japan, America and European countries to treat various conditions, such as cardiovascular and cerebrovascular disease, due to their excellent medicinal values. A novel fructose-bisphosphate aldolase gene (designated as SmFBA, GenBank accession no. FJ540907) was cloned from S. miltiorrhiza for the first time. The full-length cDNA of SmFBA was 1 390 bp with an open reading frame (ORF) of 1 065 bp which encoding a protein of 355 amino acid residues. The deduced protein had isoelectric point (pI) of 5.60 and a calculated molecular weight of about 37.78 ku. The deduced amino acid sequence of SmFBA gene shared high homology and identity with other plant FBAs. The SmFBA genomic DNA sequence was also obtained, revealing SmFBA had three exons and two introns. Southern-blot analysis indicated that SmFBA was a low-copy gene in S. miltiorrhiza genomic. Real-time quantitative PCR analysis showed that SmFBA expressed constitutively in all tested organs, with the highest expression level in roots. In addition, the recombinant SmFBA protein has enzyme activity in E. coli and could improve the high-salinity stress tolerance of E. coli. The successful isolation of the SmFBA gene will be helpful for studying EMP pathway in the near future.
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    [9] Effenberger F, Straub A. A novel convenient preparation of dihydroxyacetonphosphate and its use in enzymatic aldol reactions[J]. Tetrahedron Lett, 1987, 28:1641.
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    [14] Kai GY, Jiang JH, Zhao DL, et al. Isolation and expression profile analysis of a new cDNA encoding 5-alpha-taxadienol-10-beta-hydroxylase from Taxus media[J]. J Plant Biochem Biotechnol,2006, 15:1.
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    [20] Fan W, Zhang Z, Zhang Y. Cloning and molecular characterization of fructose-1,6-bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum[J]. Plant Cell Rep,2009, 28(6):975.
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Cloning and molecular characterization of fructose-1, 6-bisphosphate aldolase gene from Salvia miltiorrhiza

doi: 10.3969/j.issn.1006-0111.2014.01.009
  • Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2012-12-19
  • Rev Recd Date: 2013-10-29

Abstract: Salvia miltiorrhiza (Bung), a famous medicinal plant, is widely used in China, Japan, America and European countries to treat various conditions, such as cardiovascular and cerebrovascular disease, due to their excellent medicinal values. A novel fructose-bisphosphate aldolase gene (designated as SmFBA, GenBank accession no. FJ540907) was cloned from S. miltiorrhiza for the first time. The full-length cDNA of SmFBA was 1 390 bp with an open reading frame (ORF) of 1 065 bp which encoding a protein of 355 amino acid residues. The deduced protein had isoelectric point (pI) of 5.60 and a calculated molecular weight of about 37.78 ku. The deduced amino acid sequence of SmFBA gene shared high homology and identity with other plant FBAs. The SmFBA genomic DNA sequence was also obtained, revealing SmFBA had three exons and two introns. Southern-blot analysis indicated that SmFBA was a low-copy gene in S. miltiorrhiza genomic. Real-time quantitative PCR analysis showed that SmFBA expressed constitutively in all tested organs, with the highest expression level in roots. In addition, the recombinant SmFBA protein has enzyme activity in E. coli and could improve the high-salinity stress tolerance of E. coli. The successful isolation of the SmFBA gene will be helpful for studying EMP pathway in the near future.

Tan Hexin, Liu Ying, Zhang Lei. Cloning and molecular characterization of fructose-1, 6-bisphosphate aldolase gene from Salvia miltiorrhiza[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 35-41,48. doi: 10.3969/j.issn.1006-0111.2014.01.009
Citation: Tan Hexin, Liu Ying, Zhang Lei. Cloning and molecular characterization of fructose-1, 6-bisphosphate aldolase gene from Salvia miltiorrhiza[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 35-41,48. doi: 10.3969/j.issn.1006-0111.2014.01.009
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