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ZHANG Jianhua, XU Yueming, HE Yuqiong, SONG Hongtao, DU Juan, ZHANG Qiaoyan. Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 328-333. doi: 10.3969/j.issn.1006-0111.2017.04.010
Citation:
YAN Jun, TANG Dahai, XUAN Weidong, FAN Zhengping, HU Shuigen. Therapeutic effects of Thesium chinensis extracts on IgAN rats[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 28-31,35. doi: 10.3969/j.issn.1006-0111.2015.01.007
Therapeutic effects of Thesium chinensis extracts on IgAN rats
No.411 Hospital of PLA, Shanghai 200434, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 333004, China
2.
No.411 Hospital of PLA, Shanghai 200434, China
Received Date: 2013-12-04
Rev Recd Date:
2014-07-03
Abstract
Objective To evaluate the therapeutic effects of Thesium chinensis Turcz extracts on IgA nephropathy (IgAN) rats. Methods The hot water extracted solution of Thesium chinensis Turcz flow through the AB-8 macroreticular resin column repeatedly,then being eluted with different contents of ethanol to get four part of extract.The IgAN model of SD rats were established by oral intake of bovine serum albumin(BSA,400 mg/kg) together with injection of lipopolysaccharide(LPS) and CCl4.Beginning with 8th week,the positive group(LGT,10 mg/kg),water extract group(TT,1.0 g/kg),water soluble group(TTW,0.4 g/kg),high polarity group(TT20,0.4 g/kg) and medium polarity group(TT50,0.4 g/kg) rats were intragastric administrated drug daily,the normal group control and model control group rats were given with saline(n=8).During the next 5 weeks treatment process,the 24 h urine volume,24 h urine protein concentration,and quantity of erythrocyte in urine were observed.The levels of TP,ALP,ALT,AST,Scr and BUN in serum were determined.The expression of IgA deposition was measured by immunofluorescence staining,and the pathological changes of kidney tissue were assayed after the therapy process. Results After 5 weeks therapy,the 24 h urine protein concentration,the quantity of erythrocyte in urine,and the level of Scr and BUN of TT and TT50 groups were significantly lower than model control.The fluorescence intensities in the mesangial area were weaker than model control.Also,the degrees of renal capsule expansion and mesangial matrix proliferations,and the kidney tubules damage of TT and TT50 groups were weaker than those of model control. Conclusion The water extract and its medium polarity fraction of Thesium chinensis Turcz show good therapeutic effects on IgAN rats.
Lee IIK,Kim KH,Choi SU,et al.Phytochemical constituents of Thesium chinense Turcz and their cytotoxic activities in vitro[J].Nat Prod Sci,2009,15(4):246-249.
Parveen Z,Deng Y,Saeed MK,et al.Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids,kaempferol and kaempferol-3-O-glucoside[J].Yakugaru Zasshi,2007,127(8):1275-1279.
[10]
Wang H,Zhou CL,Lei H,et al.Kaempferol:a new immunosuppressant of calcineufin[J].IUBMB Life,2008,60(8):549-554.
[11]
Okamto I,Iwaki K,Koya-Miyata S,et al.The flavonoid kaempferol suppresses the graft-versus-host reaction by inhibiting type 1 cytokine production and CD8+ T cell engraftment[J].Clin Immunol,2002,103(2):132-144.
[12]
Parveen Z,Deng YL,Muhammad KS,et al.Optimizations of conditions for recovery of Astragalin from Thesium chinense Turcz[J].J Appl Sci,2006,6(13):2829-2832.
Abstract: Objective To evaluate the therapeutic effects of Thesium chinensis Turcz extracts on IgA nephropathy (IgAN) rats. Methods The hot water extracted solution of Thesium chinensis Turcz flow through the AB-8 macroreticular resin column repeatedly,then being eluted with different contents of ethanol to get four part of extract.The IgAN model of SD rats were established by oral intake of bovine serum albumin(BSA,400 mg/kg) together with injection of lipopolysaccharide(LPS) and CCl4.Beginning with 8th week,the positive group(LGT,10 mg/kg),water extract group(TT,1.0 g/kg),water soluble group(TTW,0.4 g/kg),high polarity group(TT20,0.4 g/kg) and medium polarity group(TT50,0.4 g/kg) rats were intragastric administrated drug daily,the normal group control and model control group rats were given with saline(n=8).During the next 5 weeks treatment process,the 24 h urine volume,24 h urine protein concentration,and quantity of erythrocyte in urine were observed.The levels of TP,ALP,ALT,AST,Scr and BUN in serum were determined.The expression of IgA deposition was measured by immunofluorescence staining,and the pathological changes of kidney tissue were assayed after the therapy process. Results After 5 weeks therapy,the 24 h urine protein concentration,the quantity of erythrocyte in urine,and the level of Scr and BUN of TT and TT50 groups were significantly lower than model control.The fluorescence intensities in the mesangial area were weaker than model control.Also,the degrees of renal capsule expansion and mesangial matrix proliferations,and the kidney tubules damage of TT and TT50 groups were weaker than those of model control. Conclusion The water extract and its medium polarity fraction of Thesium chinensis Turcz show good therapeutic effects on IgAN rats.
ZHANG Jianhua, XU Yueming, HE Yuqiong, SONG Hongtao, DU Juan, ZHANG Qiaoyan. Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 328-333. doi: 10.3969/j.issn.1006-0111.2017.04.010
Citation:
YAN Jun, TANG Dahai, XUAN Weidong, FAN Zhengping, HU Shuigen. Therapeutic effects of Thesium chinensis extracts on IgAN rats[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 28-31,35. doi: 10.3969/j.issn.1006-0111.2015.01.007
Lee IIK,Kim KH,Choi SU,et al.Phytochemical constituents of Thesium chinense Turcz and their cytotoxic activities in vitro[J].Nat Prod Sci,2009,15(4):246-249.
Parveen Z,Deng Y,Saeed MK,et al.Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids,kaempferol and kaempferol-3-O-glucoside[J].Yakugaru Zasshi,2007,127(8):1275-1279.
[10]
Wang H,Zhou CL,Lei H,et al.Kaempferol:a new immunosuppressant of calcineufin[J].IUBMB Life,2008,60(8):549-554.
[11]
Okamto I,Iwaki K,Koya-Miyata S,et al.The flavonoid kaempferol suppresses the graft-versus-host reaction by inhibiting type 1 cytokine production and CD8+ T cell engraftment[J].Clin Immunol,2002,103(2):132-144.
[12]
Parveen Z,Deng YL,Muhammad KS,et al.Optimizations of conditions for recovery of Astragalin from Thesium chinense Turcz[J].J Appl Sci,2006,6(13):2829-2832.
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ZHANG Jianhua, XU Yueming, HE Yuqiong, SONG Hongtao, DU Juan, ZHANG Qiaoyan. Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 328-333. doi: 10.3969/j.issn.1006-0111.2017.04.010
ZHANG Jianhua, XU Yueming, HE Yuqiong, SONG Hongtao, DU Juan, ZHANG Qiaoyan. Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 328-333. doi: 10.3969/j.issn.1006-0111.2017.04.010
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