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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(1): 44-48

XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
Citation: XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
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The preparation and the cell uptake of polymer vesicles modified with dual ligands

doi: 10.3969/j.issn.1006-0111.2015.01.011

  • Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2014-10-11
  • Rev Recd Date: 2014-11-25
  • Objective To construct an active targeting drug delivery system- polymer vesicles(PVs),and examined the cellular uptake. Methods Maleimide-polyethylene glycol-poly(lactic-co-glycolic acid)(MAL-PEG-PLGA)was used as carrier materials to prepare PVs by self-assembling.And then PVs was modified by Tf and Tet-1(Tf/Tet-1-PVs).To evaluate its active targeting,coumarin-6 was used as a fluorescent probe to analyze cellular uptake of PVs for both BCEC and Neuro-2a cells. Results PVs was about 80 nm with rounded shape and had obvious film structure.Tf/Tet-1-PVs exhibited a significant role in promoting cellular uptake for both BCEC and Neuro-2a cells compared with control and single ligand-modified group. Conclusion PVs modified with dual ligands could promote the cell uptake for both brain capillary cells and nerve cells.
  • [1] 吴 珍,杜士明,董永成.纳米粒作为脑靶向给药系统的研究进展[J].中国药房,2007,18(13):1029-1031.
    [2] Pardridge WM.Blood-brain barrier drug targeting:the future of brain drug development[J].Mol Interv,2003,3(2):90-105.
    [3] Overmoyer B,Silverman P,Holder LW,et al.Pegylated liposomal doxorubicin and cyclophosphamide as first-line therapy for patients with metastatic or recurrent breast cancer[J].Clin Breast Cancer,2005,6(2):150-157.
    [4] Vorobiof DA, Rapoport BL,Chasen MR,et al.First line therapy with paclitaxel (Taxol) and pegylated liposomal doxorubicin (Caelyx) in patients with metastatic breast cancer:a multicentre phase II study[J].Breast,2004,13(3):219-226.
    [5] Boulikas T.Clinical overview on lipoplatin:a successful liposomal formulation of cisplatin[J].Expert Opin Investig Drugs,2009,18(8):1197-1218.
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    [7] 陆 彬. 新型给药系统聚合物泡囊及其研究进展(上)[J].中国药师,2009,12(4):439-442,446.
    [8] Cheng JJ,Teply BA,Sherifi I,et al.Formulation of functionalized PLGA-PEG nanoparticles for in vivo targeted drug delivery[J].Biomaterials,2007,28(5):869-876.
    [9] Zhang PC,Hu LJ,Yin Q,et al.Transferrin-modified c[RGDfK]-paclitaxel loaded hybrid micelle for sequential blood-brain barrier penetration and glioma targeting therapy[J].Mol Pharm,2012,9(6):1590-1598.
    [10] Kwon EJ,Lasiene J,Jacobson BE,et al.Targeted nonviral delivery vehicles to neural progenitor cells in the mouse subventricularzone[J].Biomaterials,2010,31(8):2417-2424.
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The preparation and the cell uptake of polymer vesicles modified with dual ligands

doi: 10.3969/j.issn.1006-0111.2015.01.011
  • Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2014-10-11
  • Rev Recd Date: 2014-11-25

Abstract: Objective To construct an active targeting drug delivery system- polymer vesicles(PVs),and examined the cellular uptake. Methods Maleimide-polyethylene glycol-poly(lactic-co-glycolic acid)(MAL-PEG-PLGA)was used as carrier materials to prepare PVs by self-assembling.And then PVs was modified by Tf and Tet-1(Tf/Tet-1-PVs).To evaluate its active targeting,coumarin-6 was used as a fluorescent probe to analyze cellular uptake of PVs for both BCEC and Neuro-2a cells. Results PVs was about 80 nm with rounded shape and had obvious film structure.Tf/Tet-1-PVs exhibited a significant role in promoting cellular uptake for both BCEC and Neuro-2a cells compared with control and single ligand-modified group. Conclusion PVs modified with dual ligands could promote the cell uptake for both brain capillary cells and nerve cells.

XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
Citation: XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
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