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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(1): 58-59,82

WANG Tao, ZHOU Baozhu, QIU Yan, LI Maoxing, LIU Yantong. Phenylethanoid glycosides of Pedicularis muscicola ameliorate high altitude-induced memory impairment by activating mTOR signal pathway[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 238-242. doi: 10.3969/j.issn.1006-0111.2017.03.011
Citation: WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
shu

Determination of fluoxetine hydrochloride by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.015

  • Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China

  • Received Date: 2013-05-22
  • Rev Recd Date: 2014-04-07
  • Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.
  • [1] 周亦卫,张 圩,王建平,等.抗抑郁新药——盐酸氟西汀[J].上海化工杂志,1998,23(22):28-30.
    [2] 国家药品监督管理局标准(试行).盐酸氟西汀质量标准[S].[WS-551(X-434)-98].
    [3] 吕竹芬,谢清春,申 楼.HPLC测定盐酸氟西汀胶囊的含量[J].广东药学杂志,2005,15(3):13-15.
    [4] 熊凤梅,木合塔尔·吐尔洪,木尼热·阿布都克力木,等.用毛细管电泳电化学发光法测定盐酸氟西汀的含量[J].药物分析杂志,2008,28(3):440-442.
    [5] The British Pharmacopeial Convention.British Pharmacopeia (6th ed)[S].2011:938-939.
    [6] The European Pharmacopeial Convention.European Pharmacopeia[S].(7 th ed),2011:2048-2049.
    [7] The United States Pharmacopeial Convention.U.S.Pharmacopeia[S].(35 th ed),2012:3241-3242.
    [8] 国家药典委员会.中华人民共和国药典2010年版 二部[S].北京:中国医药科技出版社,2010:194-195.
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Determination of fluoxetine hydrochloride by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.015
  • Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China
  • Received Date: 2013-05-22
  • Rev Recd Date: 2014-04-07

Abstract: Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.

WANG Tao, ZHOU Baozhu, QIU Yan, LI Maoxing, LIU Yantong. Phenylethanoid glycosides of Pedicularis muscicola ameliorate high altitude-induced memory impairment by activating mTOR signal pathway[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 238-242. doi: 10.3969/j.issn.1006-0111.2017.03.011
Citation: WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
shu

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