Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 33 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(1): 58-59,82

WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
Citation: WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
shu

Determination of fluoxetine hydrochloride by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.015

  • Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China

  • Received Date: 2013-05-22
  • Rev Recd Date: 2014-04-07
  • Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.
  • [1] 周亦卫,张 圩,王建平,等.抗抑郁新药——盐酸氟西汀[J].上海化工杂志,1998,23(22):28-30.
    [2] 国家药品监督管理局标准(试行).盐酸氟西汀质量标准[S].[WS-551(X-434)-98].
    [3] 吕竹芬,谢清春,申 楼.HPLC测定盐酸氟西汀胶囊的含量[J].广东药学杂志,2005,15(3):13-15.
    [4] 熊凤梅,木合塔尔·吐尔洪,木尼热·阿布都克力木,等.用毛细管电泳电化学发光法测定盐酸氟西汀的含量[J].药物分析杂志,2008,28(3):440-442.
    [5] The British Pharmacopeial Convention.British Pharmacopeia (6th ed)[S].2011:938-939.
    [6] The European Pharmacopeial Convention.European Pharmacopeia[S].(7 th ed),2011:2048-2049.
    [7] The United States Pharmacopeial Convention.U.S.Pharmacopeia[S].(35 th ed),2012:3241-3242.
    [8] 国家药典委员会.中华人民共和国药典2010年版 二部[S].北京:中国医药科技出版社,2010:194-195.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2496) PDF downloads(79) Cited by()

Related
Proportional views

Determination of fluoxetine hydrochloride by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.015
  • Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China
  • Received Date: 2013-05-22
  • Rev Recd Date: 2014-04-07

Abstract: Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.

WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
Citation: WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
shu

    HTML

Reference (8)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return