Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
Citation:
WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China
Received Date: 2013-05-22
Rev Recd Date:
2014-04-07
Abstract
Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.
Abstract: Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
Citation:
WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002