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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(3): 209-212

YANG Yua, WANG Hui, CAO Yingying, ZHU Zhenyu. Metabolic analysis of miconazole-treated Candida albicans by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005
Citation: YANG Yua, WANG Hui, CAO Yingying, ZHU Zhenyu. Metabolic analysis of miconazole-treated Candida albicans by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005
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Metabolic analysis of miconazole-treated Candida albicans by GC-MS

doi: 10.3969/j.issn.1006-0111.2015.03.005
  • 1.

    School of Pharmacy, Second Military Medical University, Department of Drug Analysis, Shanghai 200433 China

  • 2.

    School of Pharmacy, Second Military Medical University, New Drug Research and Develepment Center, Shanghai 200433 China

  • Received Date: 2014-10-21
  • Rev Recd Date: 2015-02-28
  • Objective To investigate the metabolism in miconazole-treated Candida albicans, search for possible biomarkers and discuss the mechanism of miconazole. Methods GC-MS was employed to determine the metabolic fingerprint of Candida albicans before and after treatment with miconazole, and the difference based on multivariate was compared by statistical analysis, the potential biomarkers were screened out and the mechanism of miconazole was discussed. Results Twenty three metabolites was screened out as potential biomarkers, and they were primarily involved in amino acid metabolism, citrate cycle, glycolysis and lipid metabolism. Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways.
  • [1] Kriengkauykiat J, Ito JI, Dadwal SS. Epidemiology and treatment approaches in management of invasive fungal infections[J]. Clin Epidemiol, 2011, 3: 175-191.
    [2] Vandenbosch D, De Canck E, Dhondt I, et al. Genomewide screening for genes involved in biofilm formation and miconazole susceptibility in Saccharomyces cerevisiae[J]. FEMS Yeast Res, 2013, 13(8): 720-730.
    [3] Zhu CX, Yan L, Wang XJ, et al. Transposition of the Zorro2 retrotransposon is activated by miconazole in Candida albicans[J]. Biol Pharm Bull, 2014, 37(1): 37-43.
    [4] 吴海棠,李 祥,王添琦,等.黄芩素作用白假丝酵母菌的GC-MS代谢组学研究[J]. 第二军医大学学报, 2013, 34 (2): 184-189.
    [5] Calahorra M, Lozano C, Sanchez NS, et al. Ketoconazole and miconazole alter potassium homeostasis in Saccharomyces cerevisiae[J]. Biochim Biophys Acta, 2011, 1808 (1): 433-445.
    [6] Sanchez-Fresneda R, Guirao-Abad JP, Arguelles A, et al. Specific stress-induced storage of trehalose, glycerol and D-arabitol in response to oxidative and osmotic stress in Candida albicans[J]. Biochem Biophys Res Commun, 2013, 430 (4): 1334-1339.
    [7] Feehily C, O'Byrne CP, Karatzas KA. Functional γ-Aminobutyrate Shunt in Listeria monocytogenes: role in acid tolerance and succinate biosynthesis[J]. Appl Environ Microbiol, 2013,79 (1): 74-80.
    [8] Cao J, Barbosa JM, Singh NK, et al. GABA shunt mediates thermotolerance in Saccharomyces cerevisiae by reducing reactive oxygen production[J]. Yeast, 2013, 30 (4): 129-144.
    [9] Kobayashi D, Kondo K, Uehara N, et al. Endogenous reactive oxygen species is an important mediator of miconazole antifungal effect[J]. Antimicrob Agents Chemother, 2002, 46 (10): 3113-3117.
    [10] Shim EH, Livi CB, Rakheja D, et al. L-2-hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer[J]. Cancer Discov, 2014, 4 (11): 1290-1298.
    [11] Rawal Y, Qiu H, Hinnebusch AG. Accumulation of a threonine biosynthetic intermediate attenuates general amino acid control by accelerating degradation of Gcn4 via Pho85 and Cdk8[J]. PLoS Genet, 2014, 10 (7): e1004534.
    [12] Pasrija R, Panwar SL, Prasad R. Multidrug transporters CaCdr1p and CaMdr1p of Candida albicans display different lipid specificities: both ergosterol and sphingolipids are essential for targeting of CaCdr1p to membrane rafts[J]. Antimicrob Agents Chemother, 2008, 52 (2): 694-704.
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Metabolic analysis of miconazole-treated Candida albicans by GC-MS

doi: 10.3969/j.issn.1006-0111.2015.03.005
  • 1. School of Pharmacy, Second Military Medical University, Department of Drug Analysis, Shanghai 200433 China
  • 2. School of Pharmacy, Second Military Medical University, New Drug Research and Develepment Center, Shanghai 200433 China
  • Received Date: 2014-10-21
  • Rev Recd Date: 2015-02-28

Abstract: Objective To investigate the metabolism in miconazole-treated Candida albicans, search for possible biomarkers and discuss the mechanism of miconazole. Methods GC-MS was employed to determine the metabolic fingerprint of Candida albicans before and after treatment with miconazole, and the difference based on multivariate was compared by statistical analysis, the potential biomarkers were screened out and the mechanism of miconazole was discussed. Results Twenty three metabolites was screened out as potential biomarkers, and they were primarily involved in amino acid metabolism, citrate cycle, glycolysis and lipid metabolism. Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways.

YANG Yua, WANG Hui, CAO Yingying, ZHU Zhenyu. Metabolic analysis of miconazole-treated Candida albicans by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005
Citation: YANG Yua, WANG Hui, CAO Yingying, ZHU Zhenyu. Metabolic analysis of miconazole-treated Candida albicans by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005
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