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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(4): 319-323

YUAN Wang, WANG Meiling, SHI Yan, CUI Lili. Preparation,formulation optimization and preliminary pharmacodynamic experiment of insulin-loaded N-trimethyl chitosan nanoparticles[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 319-323. doi: 10.3969/j.issn.1006-0111.2015.04.008
Citation: YUAN Wang, WANG Meiling, SHI Yan, CUI Lili. Preparation,formulation optimization and preliminary pharmacodynamic experiment of insulin-loaded N-trimethyl chitosan nanoparticles[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 319-323. doi: 10.3969/j.issn.1006-0111.2015.04.008
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Preparation,formulation optimization and preliminary pharmacodynamic experiment of insulin-loaded N-trimethyl chitosan nanoparticles

doi: 10.3969/j.issn.1006-0111.2015.04.008
  • 1.

    Department of Inorganic Chemistry, School of Pharmacy; Second Military Medical University, Shanghai 200433, China

  • 2.

    Department of Physics and Mathematics, College of Basic Medical Sciences; Second Military Medical University, Shanghai 200433, China

  • 3.

    College of Naval Medicine, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2015-02-10
  • Rev Recd Date: 2015-06-02
  • Objective To prepare and optimize insulin-loaded N-trimethyl chitosan nanoparticles via orthogonal design, and preliminarily study the effects on blood glucose level. Methods The insulin-loaded N-trimethyl chitosan nanoparticles(INS-NPs) were prepared by ionic gelation, and the optimal proportion was obtained with orthogonal design. The nanoparticles were characterized by transmission electron microscopy and zeta/sizer nano analyzer. The entrapment efficiency of insulin-loaded nanoparticles and the in vitro releasing characteristics were studied by HPLC. A preliminary pharmacodynamic study of the insulin-loaded nanoparticles was also carried out. Results The INS-NPs exhibited narrow distribution. The mean diameter of INS-NPs was (63.26±1.88)nm, while the entrapment efficiency was (37.92±2.11)%. After 8 hours of subcutaneous injection of the INS-NPs, the blood glucose level in diabetic rats decreased to normal level and kept stable. Conclusion The optimized insulin-loaded nanoparticles had homogeneous spherical shape, small size, and could be a new approach of administration for insulin.
  • [1] Unwin N, Gan D, Whiting D. The IDF diabetes atlas: providing evidence, raising awareness and promoting action [J]. Diabetes Res Clin Pract, 2010, 87(1): 2-3.
    [2] 韩丽娜,尹丽芳,赵月芳,等.胰岛素/维生素B12-透明质酸纳米粒的制备及口服给药体内外性质的评价[J].中山大学学报,2012,33(5): 597-602.
    [3] 欧 歌,廖德华,张 凯,等.采用离子交联法制备壳聚糖胰岛素纳米粒的研究进展[J].中南药学,2013,11(2): 111-119.
    [4] Avadi MR, Sadeghi AM, Mohammadpour N, et al. Preparation and characterization of insulin nanoparticles using chitosan and arabic gum with ionic gelation method[J]. Nanomedicine, 2010, 6(1): 58-63.
    [5] 曹 姗,胡凯莉,刘 梅,等.多西紫杉醇口服磷脂纳米粒的制备与处方优化[J].中成药,2013, 35(9): 1896-1900.
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Preparation,formulation optimization and preliminary pharmacodynamic experiment of insulin-loaded N-trimethyl chitosan nanoparticles

doi: 10.3969/j.issn.1006-0111.2015.04.008
  • 1. Department of Inorganic Chemistry, School of Pharmacy; Second Military Medical University, Shanghai 200433, China
  • 2. Department of Physics and Mathematics, College of Basic Medical Sciences; Second Military Medical University, Shanghai 200433, China
  • 3. College of Naval Medicine, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2015-02-10
  • Rev Recd Date: 2015-06-02

Abstract: Objective To prepare and optimize insulin-loaded N-trimethyl chitosan nanoparticles via orthogonal design, and preliminarily study the effects on blood glucose level. Methods The insulin-loaded N-trimethyl chitosan nanoparticles(INS-NPs) were prepared by ionic gelation, and the optimal proportion was obtained with orthogonal design. The nanoparticles were characterized by transmission electron microscopy and zeta/sizer nano analyzer. The entrapment efficiency of insulin-loaded nanoparticles and the in vitro releasing characteristics were studied by HPLC. A preliminary pharmacodynamic study of the insulin-loaded nanoparticles was also carried out. Results The INS-NPs exhibited narrow distribution. The mean diameter of INS-NPs was (63.26±1.88)nm, while the entrapment efficiency was (37.92±2.11)%. After 8 hours of subcutaneous injection of the INS-NPs, the blood glucose level in diabetic rats decreased to normal level and kept stable. Conclusion The optimized insulin-loaded nanoparticles had homogeneous spherical shape, small size, and could be a new approach of administration for insulin.

YUAN Wang, WANG Meiling, SHI Yan, CUI Lili. Preparation,formulation optimization and preliminary pharmacodynamic experiment of insulin-loaded N-trimethyl chitosan nanoparticles[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 319-323. doi: 10.3969/j.issn.1006-0111.2015.04.008
Citation: YUAN Wang, WANG Meiling, SHI Yan, CUI Lili. Preparation,formulation optimization and preliminary pharmacodynamic experiment of insulin-loaded N-trimethyl chitosan nanoparticles[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 319-323. doi: 10.3969/j.issn.1006-0111.2015.04.008
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