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ZHANG Guangyu, DU Jing, LIU Mengzhen, ZHU Danni, YAN Hui, LIU Chong. Protective effect and mechanisms of neostigmine in combination with anisodamine against pulmonary oxygen toxicity[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(10): 433-438, 444. doi: 10.12206/j.issn.2097-2024.202310049
Citation:
XIAO Jinbao, JIANG Zongwen, SUN Dan, XIAO Qiusheng, WANG Shuang. Determination of the rosmarinic acid in Xiaokang capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 555-556,560. doi: 10.3969/j.issn.1006-0111.2015.06.020
Determination of the rosmarinic acid in Xiaokang capsule by HPLC
Department of Pharmacy, 461th Clinical Department, No.208 Hospital of PLA, Changchun 130021, China
2.
Outpatient Department of Air force Aviation University, Changchun 130021, China
Received Date: 2014-11-25
Rev Recd Date:
2015-04-03
Abstract
Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules. Methods An HPLC method was developed. The determination was performed on Agilent C18 column at 30℃ with a mobile phase composed of methanol: 0.5% formic acid (50:50) run at a flow rate of 1.0 ml/min. The detection wavelength was set at 330 nm and the injection volume was 10 μl. Results The linear range of rosmarinic acid concentrations was 2.88 to 20.1 μg/ml (r=0.999 8). The average recovery was 99.5% (RSD=0.40%,r=9). Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple, rapid, accurate and reliable has been developed.
Abstract: Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules. Methods An HPLC method was developed. The determination was performed on Agilent C18 column at 30℃ with a mobile phase composed of methanol: 0.5% formic acid (50:50) run at a flow rate of 1.0 ml/min. The detection wavelength was set at 330 nm and the injection volume was 10 μl. Results The linear range of rosmarinic acid concentrations was 2.88 to 20.1 μg/ml (r=0.999 8). The average recovery was 99.5% (RSD=0.40%,r=9). Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple, rapid, accurate and reliable has been developed.
ZHANG Guangyu, DU Jing, LIU Mengzhen, ZHU Danni, YAN Hui, LIU Chong. Protective effect and mechanisms of neostigmine in combination with anisodamine against pulmonary oxygen toxicity[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(10): 433-438, 444. doi: 10.12206/j.issn.2097-2024.202310049
Citation:
XIAO Jinbao, JIANG Zongwen, SUN Dan, XIAO Qiusheng, WANG Shuang. Determination of the rosmarinic acid in Xiaokang capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 555-556,560. doi: 10.3969/j.issn.1006-0111.2015.06.020
ZHANG Guangyu, DU Jing, LIU Mengzhen, ZHU Danni, YAN Hui, LIU Chong. Protective effect and mechanisms of neostigmine in combination with anisodamine against pulmonary oxygen toxicity[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(10): 433-438, 444. doi: 10.12206/j.issn.2097-2024.202310049
ZHANG Guangyu, DU Jing, LIU Mengzhen, ZHU Danni, YAN Hui, LIU Chong. Protective effect and mechanisms of neostigmine in combination with anisodamine against pulmonary oxygen toxicity[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(10): 433-438, 444. doi: 10.12206/j.issn.2097-2024.202310049
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