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LUO Chuan, LI Jing, ZHANG Wannian, MIAO Zhenyuan. Discovery of bepridil as a valuable lead compound with potent p53-MDM2 inhibitory activity[J]. Journal of Pharmaceutical Practice and Service, 2021, 39(2): 126-129. doi: 10.12206/j.issn.1006-0111.202009031
Citation:
XIANG Jingjie, ZHONG Yanqiang, LU Yiming, LU Ying. Preparation of DNA-loaded chitosan nanoparticle vaccine[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 19-23,40. doi: 10.3969/j.issn.1006-0111.2016.01.006
Preparation of DNA-loaded chitosan nanoparticle vaccine
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Received Date: 2015-08-24
Rev Recd Date:
2015-11-16
Abstract
Objective To study and optimize the preparation condition of pVAX1-wapA-loaded nanoparticles and determine the transfection efficiency. Methods The related effects of the crucial factors for the formation of nanoparticles: concentration of chitosan and TPP, pH value, N/P ratio were studied by single-factor experiment, with nanoparticles size and zeta potential as index. Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano-carrier. Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle(CS),(219.2±18.2) nm;quaternary ammonium chitosan nanoparticles(CSTM),(222.5±15.6) nm. Zeta potential of CS and CSTM was (24.7±3.5) mV, (19.6±1.2) mV and encapsulation efficiency was 91.24%, 87.66%,respectively.CSTM nanoparticle could enhance cellular uptake of pVAX1-wapA obviously. Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector.
Erbacher P,Zou S, Bettinger T,et al. Chitosan-based vector/DNA complexes for gene delivery: Biophysical Characteristics and Transfection Ability[J].Pharm Res,1998,15(9):1332-1339.
[6]
Lee M,Nah JW,Kwon Y,et al. Water-soluble and low molecular weight chitosan-based plasmid DNA delivery[J]. Pharm Res,2001,18(4):427-431.
[7]
Ishii T,Okahata Y,Sato T. Mechanism of cell transfection with plasmid/chitosan complexes[J]. Biochim Biophys Acta,2001,1514(1):51-64.
[8]
Huang M,Fong CW,Khor E,et al. Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation[J]. J Control Release,2005,106(3):391-406.
Qian F,Cui F,Ding J,et al. Chitosan graft copolymer nanoparticles for oral protein drug delivery: preparation and characterization[J].Biomacromolecules,2006,7(10):2722-2727.
Mao HQ,Roy K,Troung-Le VL,et al.Chitosan-DNA nano-particles as gene carriers: synthesis, characterization and transfection efficiency [J]. J Control Release,2001,70 (3): 399-421.
Abstract: Objective To study and optimize the preparation condition of pVAX1-wapA-loaded nanoparticles and determine the transfection efficiency. Methods The related effects of the crucial factors for the formation of nanoparticles: concentration of chitosan and TPP, pH value, N/P ratio were studied by single-factor experiment, with nanoparticles size and zeta potential as index. Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano-carrier. Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle(CS),(219.2±18.2) nm;quaternary ammonium chitosan nanoparticles(CSTM),(222.5±15.6) nm. Zeta potential of CS and CSTM was (24.7±3.5) mV, (19.6±1.2) mV and encapsulation efficiency was 91.24%, 87.66%,respectively.CSTM nanoparticle could enhance cellular uptake of pVAX1-wapA obviously. Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector.
LUO Chuan, LI Jing, ZHANG Wannian, MIAO Zhenyuan. Discovery of bepridil as a valuable lead compound with potent p53-MDM2 inhibitory activity[J]. Journal of Pharmaceutical Practice and Service, 2021, 39(2): 126-129. doi: 10.12206/j.issn.1006-0111.202009031
Citation:
XIANG Jingjie, ZHONG Yanqiang, LU Yiming, LU Ying. Preparation of DNA-loaded chitosan nanoparticle vaccine[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 19-23,40. doi: 10.3969/j.issn.1006-0111.2016.01.006
Erbacher P,Zou S, Bettinger T,et al. Chitosan-based vector/DNA complexes for gene delivery: Biophysical Characteristics and Transfection Ability[J].Pharm Res,1998,15(9):1332-1339.
[6]
Lee M,Nah JW,Kwon Y,et al. Water-soluble and low molecular weight chitosan-based plasmid DNA delivery[J]. Pharm Res,2001,18(4):427-431.
[7]
Ishii T,Okahata Y,Sato T. Mechanism of cell transfection with plasmid/chitosan complexes[J]. Biochim Biophys Acta,2001,1514(1):51-64.
[8]
Huang M,Fong CW,Khor E,et al. Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation[J]. J Control Release,2005,106(3):391-406.
Qian F,Cui F,Ding J,et al. Chitosan graft copolymer nanoparticles for oral protein drug delivery: preparation and characterization[J].Biomacromolecules,2006,7(10):2722-2727.
Mao HQ,Roy K,Troung-Le VL,et al.Chitosan-DNA nano-particles as gene carriers: synthesis, characterization and transfection efficiency [J]. J Control Release,2001,70 (3): 399-421.
LUO Chuan, LI Jing, ZHANG Wannian, MIAO Zhenyuan. Discovery of bepridil as a valuable lead compound with potent p53-MDM2 inhibitory activity[J]. Journal of Pharmaceutical Practice and Service, 2021, 39(2): 126-129. doi: 10.12206/j.issn.1006-0111.202009031
LUO Chuan, LI Jing, ZHANG Wannian, MIAO Zhenyuan. Discovery of bepridil as a valuable lead compound with potent p53-MDM2 inhibitory activity[J]. Journal of Pharmaceutical Practice and Service, 2021, 39(2): 126-129. doi: 10.12206/j.issn.1006-0111.202009031
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