Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 34 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(1): 59-61

GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
Citation: GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
shu

Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC

doi: 10.3969/j.issn.1006-0111.2016.01.016

  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China

  • Received Date: 2015-06-20
  • Rev Recd Date: 2015-10-30
  • Objective To establish the method for determination content of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05 mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivation agent, respectively. Results The method showed a good linearity in the range of 0.006-0.030 mg/ml (r=0.999 3) for glycine. The average recovery was 101.4%, and the RSD was 0.14% (n=9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in the pharmaceutical of human coagulation factor Ⅷ.
  • [1] Schwarzinger I,Pabinger I,Kominger C,et al.Incidence of inhibitors in patients with severe and moderate hemophilia a treated with factor Ⅷ concentrates[J].Am J Hematol,2006,24(3):241-245.
    [2] Van DK, Van Der Bom JG, Lenting PJ, et al. Facter Ⅷ half-life and clinical phenotype of sever hemophilia A[J]. Haematologica, 2005,90(4):494-498.
    [3] 赵艳华, 马 平, 卜凤荣. 凝血因子分子生物学研究进展[J]. 生物技术通讯,1999,10(2):158-160.
    [4] 孙东坡,胡一桥.蛋白质冷冻干燥制品中的保护剂及其保护机制[J].药学进展,2003,27(4):202-205.
    [5] 李 瑜,江 勇,李 爽.反相高效液相色谱法测定发酵液中L-精氨酸含量[J]. 工业微生物,2004,34(3):32-34.
    [6] 尉小平,冉铁成,王 坚,等.柱前衍生反相高效液相色谱法测定冻干人纤维蛋白原制品中盐酸精氨酸和甘氨酸的含量[J]. 中国生物制品学杂志,2007,20(8):609-611.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(3149) PDF downloads(111) Cited by()

Related
Proportional views

Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC

doi: 10.3969/j.issn.1006-0111.2016.01.016
  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China
  • Received Date: 2015-06-20
  • Rev Recd Date: 2015-10-30

Abstract: Objective To establish the method for determination content of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05 mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivation agent, respectively. Results The method showed a good linearity in the range of 0.006-0.030 mg/ml (r=0.999 3) for glycine. The average recovery was 101.4%, and the RSD was 0.14% (n=9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in the pharmaceutical of human coagulation factor Ⅷ.

GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
Citation: GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
shu

    HTML

Reference (6)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return