Determination of baicalin in Biyanling capsule by HPLC

GUO Liangjun; SUN Liang; ZHENG Wei; LU Ping; FU Mingqiang; GUO Jianbin

doi:10.3969/j.issn.1006-0111.2017.01.015

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To develop a HPLC method for determination of baicalin. Methods The separation was carried out on a Waters XBridge C₁₈ column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 0.8% formic acid (25:75), the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the injection volume was 10 μl. Results The linearity was obtained over 1.25-40 μg/ml(r=0.999 9) for baicalin. The RSD of precision were less than 2%. The average recovery was between 95% and 100%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Biyanling capsule.

Determination of baicalin in Biyanling capsule by HPLC

No. 98 Hospital of PLA, Huzhou 313000, China

Received Date: 2015-03-13

Rev Recd Date: 2015-07-12

Key words:

Abstract: Objective To develop a HPLC method for determination of baicalin. Methods The separation was carried out on a Waters XBridge C₁₈ column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 0.8% formic acid (25:75), the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the injection volume was 10 μl. Results The linearity was obtained over 1.25-40 μg/ml(r=0.999 9) for baicalin. The RSD of precision were less than 2%. The average recovery was between 95% and 100%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Biyanling capsule.

HTML

Reference (10)

[1]	陆萍,谭兴起,郭良君,等.正交设计优选鼻炎灵胶囊的提取工艺[J].世界中西医杂志,2012,7(2):112-114.
[2]	Li BQ, Fu T, Dongyan Y, et al. Flavonoid baicalin inhibits HIV-1 infection at the level of viral entry[J].Biochem Biophys Res Commun, 2000,276(2):534-538.
[3]	Cao YG,Mao XY, Sun CY, et al. Baicalin attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-oxidative and anti-apoptotic pathways[J].Brain Res Bull, 2011,85(6):396-402.
[4]	雷芳.黄芩苷药理作用研究进展[J].中国药业,2010,19(15):87-90.
[5]	邵世峰,刘雪萍,孙婉蓉,等. 黄芩苷对结核分枝杆菌抑菌作用的初步研究[J].中国实验方剂学杂志,2012,18(4):763-765.
[6]	潘永利. 黄芩苷对急性肺损伤大鼠的保护作用[J].中国实验方剂学杂志,2013,19(3):238-240.
[7]	赵亮,王新霞,吕磊,等. HPLC法测定清肝散结颗粒中黄芩苷、汉黄芩苷、野黄芩苷、黄芩素及汉黄芩素[J].中成药,2014,36(2):313-318.
[8]	费超,陈然,曹杰,等. HPLC测定芩翘抗感颗粒中黄芩苷的含量[J].中国实验方剂学杂志,2011,17(7):63-65.
[9]	李金玉. 复方鱼腥草片中绿原酸、黄芩苷的测定及指纹图谱的建立[J]. 中国实验方剂学杂志,2014,20(12):65-69.
[10]	国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:282-283.

Message Board

Determination of baicalin in Biyanling capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2017.01.015

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views