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LU Jingrong, LIU Yawei, DAI Ying, LIN Houwen, YANG Fan. Study on the secondary metabolites from a polar marine sponge associated actinomyces Streptomyces sp. LHW11-07[J]. Journal of Pharmaceutical Practice and Service, 2022, 40(6): 499-504. doi: 10.12206/j.issn.2097-2024.202206081
Citation:
CHEN Song, WANG Yujie, WANG Huan, YUAN Yongfang. Preparation of the etoposide chitosan micelle and the effect of chitosan on etoposide intestinal absorption[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 243-247. doi: 10.3969/j.issn.1006-0111.2017.03.012
Preparation of the etoposide chitosan micelle and the effect of chitosan on etoposide intestinal absorption
Department of Pharmacy, Shanghai Ninth People's Hospital, Shanghai 201999, China
Received Date: 2016-05-15
Rev Recd Date:
2016-11-13
Abstract
Objective To prepare the etoposide chitosan micelle, and investigate the effect of chitosan on etoposide intestinal absorption. Methods The etoposide chitosan micelle was prepared by dialysis. The drug encapsulation efficiency and drug loading efficiency were determined by HPLC. The intestine in rats was cannulated for in situ recirculation. The effects of different chitosan doses on the intestinal drug absorption and the effects of chitosan on the drug absorption at different intestinal locations were studied. Results The average particle size of etoposide chitosan micelle was 139.5 nm. The multi-dispersion coefficient was 0.569. The standard curve of etoposide was A = 8 436.8 C-4 963.8,r=1.000 0. The intra-and inter-day precision values meetthe requirement. The drug encapsulation efficiency was (47.3±2.84)% and drug loading efficiency was (1.10±1.27)%. With the increase of the chitosan concentration, the absorption capacity of the unit area in the whole intestine was increased in different degrees. Chitosan exhibits its effects on etoposide absorptionat different intestinal sections in the following order: ileum >jejunum >duodenum. Conclusion Chitosan promoted etoposide absorption induodenum, jejunum and ileum, especially in jejunum and ileum.
Li M, Si L, Pan H, et al. Excipients enhance intestinal absorption of ganciclovir by P-gp inhibition: assessed in vitro by everted gut sac and in situ by improved intestinal perfusion [J]. Int J Pharm, 2011, 403(1-2):37-45.
[2]
Choo E, Leake B, Wandel C, et al. Pharmacological inhibition of P-GP transport enhances the distribution of HIV-1 protease inhibitors into brain and testes[J]. Drug Metab Dispos, 2000, 28(6): 655-660.
[3]
Illum L.Chitosan and its use as a pharmaceutical excipient[J].Pharm Res,1998,15(9): 1326-1331.
[4]
Kotze AR,Lueben HL,Leeuw BJD,et al.N-trimethyl chitosan chloride as a potential absorption enhancer across mucosal surface:in vitro evaluation in intestinal epithelial cells(Caco-2)[J].Pharm Res,1997,14(9):1197-1202.
Polnok A, Verhoef JC, Borchard G,et al.In vitro evaluation of intestinal absorption of desmopressin using drug-delivery systems based on superporous hydrogels[J].Int J Pharm,2004,269(2):303-310.
[8]
Carreno-Gomez B,Duncan R. Evaluation of the biological properties of soluble chitosan and chitosan mocrospheres[J].Int J Pharm,1997,148(2):231-240.
Abstract: Objective To prepare the etoposide chitosan micelle, and investigate the effect of chitosan on etoposide intestinal absorption. Methods The etoposide chitosan micelle was prepared by dialysis. The drug encapsulation efficiency and drug loading efficiency were determined by HPLC. The intestine in rats was cannulated for in situ recirculation. The effects of different chitosan doses on the intestinal drug absorption and the effects of chitosan on the drug absorption at different intestinal locations were studied. Results The average particle size of etoposide chitosan micelle was 139.5 nm. The multi-dispersion coefficient was 0.569. The standard curve of etoposide was A = 8 436.8 C-4 963.8,r=1.000 0. The intra-and inter-day precision values meetthe requirement. The drug encapsulation efficiency was (47.3±2.84)% and drug loading efficiency was (1.10±1.27)%. With the increase of the chitosan concentration, the absorption capacity of the unit area in the whole intestine was increased in different degrees. Chitosan exhibits its effects on etoposide absorptionat different intestinal sections in the following order: ileum >jejunum >duodenum. Conclusion Chitosan promoted etoposide absorption induodenum, jejunum and ileum, especially in jejunum and ileum.
LU Jingrong, LIU Yawei, DAI Ying, LIN Houwen, YANG Fan. Study on the secondary metabolites from a polar marine sponge associated actinomyces Streptomyces sp. LHW11-07[J]. Journal of Pharmaceutical Practice and Service, 2022, 40(6): 499-504. doi: 10.12206/j.issn.2097-2024.202206081
Citation:
CHEN Song, WANG Yujie, WANG Huan, YUAN Yongfang. Preparation of the etoposide chitosan micelle and the effect of chitosan on etoposide intestinal absorption[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 243-247. doi: 10.3969/j.issn.1006-0111.2017.03.012
Li M, Si L, Pan H, et al. Excipients enhance intestinal absorption of ganciclovir by P-gp inhibition: assessed in vitro by everted gut sac and in situ by improved intestinal perfusion [J]. Int J Pharm, 2011, 403(1-2):37-45.
[2]
Choo E, Leake B, Wandel C, et al. Pharmacological inhibition of P-GP transport enhances the distribution of HIV-1 protease inhibitors into brain and testes[J]. Drug Metab Dispos, 2000, 28(6): 655-660.
[3]
Illum L.Chitosan and its use as a pharmaceutical excipient[J].Pharm Res,1998,15(9): 1326-1331.
[4]
Kotze AR,Lueben HL,Leeuw BJD,et al.N-trimethyl chitosan chloride as a potential absorption enhancer across mucosal surface:in vitro evaluation in intestinal epithelial cells(Caco-2)[J].Pharm Res,1997,14(9):1197-1202.
Polnok A, Verhoef JC, Borchard G,et al.In vitro evaluation of intestinal absorption of desmopressin using drug-delivery systems based on superporous hydrogels[J].Int J Pharm,2004,269(2):303-310.
[8]
Carreno-Gomez B,Duncan R. Evaluation of the biological properties of soluble chitosan and chitosan mocrospheres[J].Int J Pharm,1997,148(2):231-240.
LU Jingrong, LIU Yawei, DAI Ying, LIN Houwen, YANG Fan. Study on the secondary metabolites from a polar marine sponge associated actinomyces Streptomyces sp. LHW11-07[J]. Journal of Pharmaceutical Practice and Service, 2022, 40(6): 499-504. doi: 10.12206/j.issn.2097-2024.202206081
LU Jingrong, LIU Yawei, DAI Ying, LIN Houwen, YANG Fan. Study on the secondary metabolites from a polar marine sponge associated actinomyces Streptomyces sp. LHW11-07[J]. Journal of Pharmaceutical Practice and Service, 2022, 40(6): 499-504. doi: 10.12206/j.issn.2097-2024.202206081
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