Qualitative identification and quantitative determination of Fufang Shenghua granules

CHEN Hua; YU Botao; JIN Weihua; PU Zhiqiang; SONG Zonghui; FAN Kaihua

doi:10.3969/j.issn.1006-0111.2017.03.014

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Objective To improve quality standard of Fufang Shenghua granules. Methods TLC was used to identify chief components in the preparation, Radix et Rhizoma Glycyrrhizae and Salvia Miltiorrhiza. HPLC was applied to identify Amarogentin and to determine the content of Salvianolic acid B. Salvianolic acid B assay was performed on Agilent HC-C₁₈(4.6 mm×250 mm, 5 μm) column with Acetonitrile-0.1% phosphoric acid (23:77)as mobile phase. The flow rate was 1.0 ml/min. The column temperature was 30 ℃. The detection wavelength was set at 286 nm. Results The spots on TLC were fairly clear with good separation. There was no interference from the negative control samples. However, HPLC was a more accurate, reliable and objective method for qualitative identification. Salvianolic acid B showed a good linear correlation in the range of 1.56~49.92 μg/ml (r=0.999 9). The average recovery was 100.07%, RSD 1.61% (n=9). Conclusion A simple, accurate and reliable method was developed for the quality control of Fufang Shenghua granules.

Qualitative identification and quantitative determination of Fufang Shenghua granules

Department of Pharmacy, General Hospital of Chengdu Military Command, Chengdu 610083, China

Received Date: 2016-01-26

Rev Recd Date: 2016-07-04

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Abstract: Objective To improve quality standard of Fufang Shenghua granules. Methods TLC was used to identify chief components in the preparation, Radix et Rhizoma Glycyrrhizae and Salvia Miltiorrhiza. HPLC was applied to identify Amarogentin and to determine the content of Salvianolic acid B. Salvianolic acid B assay was performed on Agilent HC-C₁₈(4.6 mm×250 mm, 5 μm) column with Acetonitrile-0.1% phosphoric acid (23:77)as mobile phase. The flow rate was 1.0 ml/min. The column temperature was 30 ℃. The detection wavelength was set at 286 nm. Results The spots on TLC were fairly clear with good separation. There was no interference from the negative control samples. However, HPLC was a more accurate, reliable and objective method for qualitative identification. Salvianolic acid B showed a good linear correlation in the range of 1.56~49.92 μg/ml (r=0.999 9). The average recovery was 100.07%, RSD 1.61% (n=9). Conclusion A simple, accurate and reliable method was developed for the quality control of Fufang Shenghua granules.

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Reference (9)

[1]	国家药典委员会.中华人民共和国药典 2010年版一部[S].北京:中国医药科技出版社,2010:282.
[2]	黄可婧,王丽娟.附子理中片的质量标准研究[J].天津药学,2014,26(5):16-20.
[3]	高森,李正,王志涛,等.荡石胶囊定性定量方法研究[J].中南药学,2013,11(11):862-855.
[4]	陈华,范开华,于波涛,等.胃病康颗粒的质量标准研究[J].药学服务与研究,2012,12(3):230-232.
[5]	尹华,章建华,张春霞,等.芪参健骨颗粒的薄层鉴别研究[J].浙江中医药大学学报,2011,35(1):79-81.
[6]	李翔,刘皈阳,马建丽,等.HPLC法测定麻杏口服液中苦杏仁苷的含量[J].解放军药学学报,2013,29(1):57-59.
[7]	刘学杰,车洪勇.通络祛瘀胶囊中苦杏仁苷的含量测定[J].中国医院药学杂志,2013,33(16):1370-1371.
[8]	肖作奇,欧阳波,潘涛,等.高效液相色谱法测定盆炎灵合剂中的阿魏酸、延胡索乙素、芍药苷、丹酚酸B及原儿茶醛含量[J].中南药学,2014,12(12):1225-1228.
[9]	谢琳,庄满贤,王亚琦,等.HPLC 法同时测定养心宁神丸中丹参酮ⅡA 和丹酚酸B 的含量[J].广东药学院学报,2015,31(1):58-61.

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Qualitative identification and quantitative determination of Fufang Shenghua granules

doi: 10.3969/j.issn.1006-0111.2017.03.014

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