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Volume 35 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(4): 359-361

YANG Mei, LI Junyuan, WU Jiabin, YU Qiuping, WEI Minling, WEI Hongyan. Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 359-361. doi: 10.3969/j.issn.1006-0111.2017.04.018
Citation: YANG Mei, LI Junyuan, WU Jiabin, YU Qiuping, WEI Minling, WEI Hongyan. Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 359-361. doi: 10.3969/j.issn.1006-0111.2017.04.018
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Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.04.018

  • PuraPharm (Nanning) Pharmaceutical Company Limited, Nanning 530007, China

  • Received Date: 2016-12-21
  • Rev Recd Date: 2017-03-10
  • Objective To establish a HPLC method for determination of madecassoside and asiaticoside in Centella asiatica formula granules. Methods Chromatographic separation was performed on Ultimate AQ-C18 column(4.6 mm×250 mm,5 μm) with mobile phase of acetonitrile(A)-2 mmol/L β-cyclodextrin(0~30 min:21% A→23% A; 30~60 min:23% A→25% A).The flow rate was set at 1.0 ml/min, the column temperature at 30 ℃ and detection wavelength at 205 nm. Results Madecassoside and asiaticoside showed good linearity (r>0.9995) in the ranges of 0.187 7~3.754 μg and 0.184 3~3.686 μg respectively. The specificity, repeatability, precision,recovery and stability were satisfied to the method validation requirements of China Pharmacopoeia. Conclusion The method can determine madecassoside and asiaticoside in Centella asiatica formula granules.
  • [1] 张红梅,宋景政,谭红胜,等.从汤剂到颗粒剂:中药配方颗粒20年回顾与展望[J].世界科学技术:中医药现代化,2012,14(4):1740-1753.
    [2] 国家药典委员会.中华人民共和国药典2015年版(一部)[S].北京:中国医药科技出版社,2015:283-284.
    [3] 张蕾磊,王海生,姚庆强,等.积雪草化学成分研究[J].中草药,2005,36(12):1761-1763.
    [4] 曹金华,谷 莉,吕 磊,等.HPLC-ELSD法测定积雪草药材及配方颗粒剂中积雪草苷的含量[J].药学实践杂志,2013,31(5):384-385,392.
    [5] 黄 晟,谷 莉,娄子洋,等.高效液相法测定积雪草配方颗粒中积雪草苷的含量[J].药物分析杂志,2004,24(5):516-518.
    [6] 郏征伟,毛秀红,陈 钶,等.手性流动相添加剂法测定积雪草中积雪草总苷的量及其总苷特征图谱[J].中草药,2010,41(12):2070-2074.
    [7] 陈云艳,邵 燕,周 靖,等.积雪草总苷及其制剂的含量研究.Ⅱ.积雪草总苷中积雪草苷、羟基积雪草苷和积雪草苷B含量的HPLC法测定[J].中国医药工业杂志,2010,41(4):276-279.
    [8] 陈云艳,邵 燕,欧阳丹薇,等.积雪草总苷及其制剂的含量研究.Ⅳ.积雪草苷B、羟基积雪草苷工作对照品的制备[J].中国医药工业杂志,2014,45(5):479-481.
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Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.04.018
  • PuraPharm (Nanning) Pharmaceutical Company Limited, Nanning 530007, China
  • Received Date: 2016-12-21
  • Rev Recd Date: 2017-03-10

Abstract: Objective To establish a HPLC method for determination of madecassoside and asiaticoside in Centella asiatica formula granules. Methods Chromatographic separation was performed on Ultimate AQ-C18 column(4.6 mm×250 mm,5 μm) with mobile phase of acetonitrile(A)-2 mmol/L β-cyclodextrin(0~30 min:21% A→23% A; 30~60 min:23% A→25% A).The flow rate was set at 1.0 ml/min, the column temperature at 30 ℃ and detection wavelength at 205 nm. Results Madecassoside and asiaticoside showed good linearity (r>0.9995) in the ranges of 0.187 7~3.754 μg and 0.184 3~3.686 μg respectively. The specificity, repeatability, precision,recovery and stability were satisfied to the method validation requirements of China Pharmacopoeia. Conclusion The method can determine madecassoside and asiaticoside in Centella asiatica formula granules.

YANG Mei, LI Junyuan, WU Jiabin, YU Qiuping, WEI Minling, WEI Hongyan. Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 359-361. doi: 10.3969/j.issn.1006-0111.2017.04.018
Citation: YANG Mei, LI Junyuan, WU Jiabin, YU Qiuping, WEI Minling, WEI Hongyan. Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 359-361. doi: 10.3969/j.issn.1006-0111.2017.04.018
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