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ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
Citation:
LI Li, FENG Jingjing, LI Tiejun, ZHANG Yuefan. Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 34-39. doi: 10.3969/j.issn.1006-0111.2018.01.007
Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China
3.
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Received Date: 2017-07-30
Rev Recd Date:
2017-09-27
Abstract
Objective To study the protective effect of Fu-Yuan-Xing-Nao decoction (FYXND) on rats with middle cerebral ischemia reperfusion injury. Methods Male Sprague-Dawley rats were randomly divided into sham, ischemia-reperfusion, and low, middle, high FYXND dose (5.5, 11, 22 g/kg) groups. Rats model was induced by 2 h of middle cerebral artery occlusion and 24 h reperfusion. The neurological deficit score of each group was evaluated. The infarct size was measured by the 2,3,5-triphenyltetra-zolium(TTC) chloride staining assay. The pathological changes of brain tissue were observed by HE staining assay. The changes of Nissl bodies were observed by Nissl staining. Tunnel staining was used to observe the apoptosis of neurons in brain. Serum levels of superoxide dismutase (SOD)and Malondialdehyde (MDA) were measured. Results Compared with the model group, neurological outcomes were improved in all three groups of low, middle and high FYXND dose (5.5, 11, 22 g/kg). Significantly reduced infarct brain volume was observed with TTC staining in all three FYXND groups. The results from HE staining assay indicated that the pathological structure of brain tissue was improved in the treatment groups. The numbers and morphology of Nissl corpuscles in the treated group were also improved based on the results of Nissl staining. Both the Tunnel staining positive cells and the rate of apoptosis were decreased. Compared with the model group, FYXND increased the rat serum SOD level and decreased the MDA level. Conclusion FYXND has protective effects on cerebral ischemia reperfusion injury in rats.
Mozaffarian D,Benjamin EJ, Go AS, et al. Heart disease and stroke statistics——2015 update:a report from the American Heart Association[J]. Circulation,2015, 131(4):e29-322.
[2]
Mozaffarian D.Dietary and policy priorities for cardiovascular disease, diabetes, and obesity:a comprehensive review[J] Circulation,2016,133(2):187-225.
Woodruff TM,Thundyil J,Tang SC,et al. Pathophysiology, treatment, and animal and cellular models of human ischemic stroke[J]. Mol Neurodegener,2011,6(1):11.
[7]
Longa EZ,Weinstein PR,Carlson S,et al. Cummins Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke,1989,20(1):84-91.
[8]
Maki T,Hayakawa K,Pham L D,et al. Biphasic mechanisms of neurovascular unit injury and protection in CNS diseases[J].CNS Neurol Disord Drug Targets,2013,12(3):302-315.
[9]
Ayuso MI,Gonzalo-Gobernado R,Montaner J.Neuroprotective diets for stroke[J]. Neurochem Int,2017,107:4-10.
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China
3. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Abstract: Objective To study the protective effect of Fu-Yuan-Xing-Nao decoction (FYXND) on rats with middle cerebral ischemia reperfusion injury. Methods Male Sprague-Dawley rats were randomly divided into sham, ischemia-reperfusion, and low, middle, high FYXND dose (5.5, 11, 22 g/kg) groups. Rats model was induced by 2 h of middle cerebral artery occlusion and 24 h reperfusion. The neurological deficit score of each group was evaluated. The infarct size was measured by the 2,3,5-triphenyltetra-zolium(TTC) chloride staining assay. The pathological changes of brain tissue were observed by HE staining assay. The changes of Nissl bodies were observed by Nissl staining. Tunnel staining was used to observe the apoptosis of neurons in brain. Serum levels of superoxide dismutase (SOD)and Malondialdehyde (MDA) were measured. Results Compared with the model group, neurological outcomes were improved in all three groups of low, middle and high FYXND dose (5.5, 11, 22 g/kg). Significantly reduced infarct brain volume was observed with TTC staining in all three FYXND groups. The results from HE staining assay indicated that the pathological structure of brain tissue was improved in the treatment groups. The numbers and morphology of Nissl corpuscles in the treated group were also improved based on the results of Nissl staining. Both the Tunnel staining positive cells and the rate of apoptosis were decreased. Compared with the model group, FYXND increased the rat serum SOD level and decreased the MDA level. Conclusion FYXND has protective effects on cerebral ischemia reperfusion injury in rats.
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
Citation:
LI Li, FENG Jingjing, LI Tiejun, ZHANG Yuefan. Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 34-39. doi: 10.3969/j.issn.1006-0111.2018.01.007
Mozaffarian D,Benjamin EJ, Go AS, et al. Heart disease and stroke statistics——2015 update:a report from the American Heart Association[J]. Circulation,2015, 131(4):e29-322.
[2]
Mozaffarian D.Dietary and policy priorities for cardiovascular disease, diabetes, and obesity:a comprehensive review[J] Circulation,2016,133(2):187-225.
Woodruff TM,Thundyil J,Tang SC,et al. Pathophysiology, treatment, and animal and cellular models of human ischemic stroke[J]. Mol Neurodegener,2011,6(1):11.
[7]
Longa EZ,Weinstein PR,Carlson S,et al. Cummins Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke,1989,20(1):84-91.
[8]
Maki T,Hayakawa K,Pham L D,et al. Biphasic mechanisms of neurovascular unit injury and protection in CNS diseases[J].CNS Neurol Disord Drug Targets,2013,12(3):302-315.
[9]
Ayuso MI,Gonzalo-Gobernado R,Montaner J.Neuroprotective diets for stroke[J]. Neurochem Int,2017,107:4-10.
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
ZHAO Anpeng, JIN Ting, WANG Rong. Protective effect of areca catechu linn ethanol extract against hypoxia in H9C2 cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 294-298. doi: 10.3969/j.issn.1006-0111.2019.04.002
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