LIU Zhiwen, CHEN Wen, YUAN Fenqian, YOU Xiaolong. Proteomic analysis of cyclopamine effect on liver regeneration[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 318-323. doi: 10.3969/j.issn.1006-0111.2018.04.007
| Citation:
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LIU Zhiwen, CHEN Wen, YUAN Fenqian, YOU Xiaolong. Proteomic analysis of cyclopamine effect on liver regeneration[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 318-323. doi: 10.3969/j.issn.1006-0111.2018.04.007
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Proteomic analysis of cyclopamine effect on liver regeneration
- 1.
Neonatal Surgery Department of Jiangxi Children's Hospital, Nanchang 330006, China
- 2.
Department of Head and Nneck Surgery, Jiangxi Oncology Hospital, Nanchang 330029, China
- Received Date: 2017-08-25
- Rev Recd Date:
2018-03-06
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Abstract
Objective To study the effect of cyclopamine on liver regeneration, and to explore its possible molecular mechanism. Methods iTRAQ-based quantitative proteomics combined with 2D liquid chromatography-tandem mass spectrometry were applied to study the protein expression of liver regeneration in rats with partial hepatectomy. Results A total of 1625 proteins were identified. 367 proteins were significantly different in the control group, cyclopamine-treated group and the partial hepatectomy group. The pathway analysis suggested that Hedgehog (HH) signaling pathway was activated in the partial hepatectomy group, and SHH, Gli2 in this pathway may participate in the regulation of liver regeneration by affecting apoptosis and energy supply. While SHH and Gli2 were down regulated in the cyclopamine group. Conclusion Cyclopamine inhibited by Hedgehog (HH) key protein signaling affects the expression of liver regeneration; Hedgehog (HH) signal pathway in the process of liver regeneration has an important role.
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