YANG Lina, ZENG Qiping, CAO Yixiang, CAI Xiaohui, CHEN Jinshan. Simultaneous determination of seven active components in compound caulis polygoni multiflori mixture by HPLC of double wavelength[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 334-337. doi: 10.3969/j.issn.1006-0111.2018.04.010
| Citation:
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YANG Lina, ZENG Qiping, CAO Yixiang, CAI Xiaohui, CHEN Jinshan. Simultaneous determination of seven active components in compound caulis polygoni multiflori mixture by HPLC of double wavelength[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 334-337. doi: 10.3969/j.issn.1006-0111.2018.04.010
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Simultaneous determination of seven active components in compound caulis polygoni multiflori mixture by HPLC of double wavelength
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The Pharmacy Department, the 175 th Hospital of PLA/the Affiliated Southeast Hospital of Xiamen University, Fujian Zhangzhou 363000, China
- Received Date: 2017-11-24
- Rev Recd Date:
2018-05-24
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Abstract
Objective To establish a double wavelength method for the simultaneous determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A in compound caulis polygoni multiflori mixture. Methods The HPLC separation was performed on an Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm), gradient eluted with the mixture of acetonitrile and 0.1% phosphoric acid as mobile phase, the flow rate was 1 ml/min, the detection wavelength was 210 nm and 320 nm, and the column temperature was 25℃. Results There were good linear relationships in the determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A (r ≥ 0.9997), with the average recovery rate of 102.33%, 100.29%, 100.18%, 99.95%, 99.23%, 101.61%, 99.28%, and the RSD were less than 3%. Conclusion The method was accurate, stable and reproducible and could be used for quality control of compound caulis polygoni multiflori mixture.
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