Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules by HPLC

HAO Zhe; WANG Lijun; ZHANG Jianbang

doi:10.3969/j.issn.1006-0111.2018.04.015

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Objective To establish a method of HPLC for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules. Methods Wondasil C₁₈(4.6 mm×250 mm,5 μm)was used at 30℃ and the acetonitrile-0.1% H₃PO₄ (5:95 ) was the mobile phase with a flow rate of 1.0 ml/min. The detection wavelength was at 215nm. Results The linear range of ephedrine hydrochloride and pseudoephedrine hydrochloride were 4.231-42.31 μg/ml(r=0.999 7)、1.187-11.87 μg/ml(r=0.999 9), the average recovery were 98.9% (RSD=0.48%,n=6) and 98.1%(RSD=0.48%,n=6). Conclusion This method was accurate and good reproducibility, which could be used for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules.

Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules by HPLC

No. 153 Hospital of PLA, Zhengzhou 450042, China

Received Date: 2017-11-23

Rev Recd Date: 2018-04-08

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Abstract: Objective To establish a method of HPLC for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules. Methods Wondasil C₁₈(4.6 mm×250 mm,5 μm)was used at 30℃ and the acetonitrile-0.1% H₃PO₄ (5:95 ) was the mobile phase with a flow rate of 1.0 ml/min. The detection wavelength was at 215nm. Results The linear range of ephedrine hydrochloride and pseudoephedrine hydrochloride were 4.231-42.31 μg/ml(r=0.999 7)、1.187-11.87 μg/ml(r=0.999 9), the average recovery were 98.9% (RSD=0.48%,n=6) and 98.1%(RSD=0.48%,n=6). Conclusion This method was accurate and good reproducibility, which could be used for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules.

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Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules by HPLC

doi: 10.3969/j.issn.1006-0111.2018.04.015

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