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Volume 36 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(4): 362-364,384

WANG Chongqing, MA Ningning, YANG Chao, LIU Shihao, ZHU Ju, ZHENG Canhui. Research progress in the catalytic rearrangement reaction of benzyl phenyl ethers[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 486-489. doi: 10.3969/j.issn.1006-0111.2015.06.002
Citation: YAO Jiajia, YAN Chen. Glycine and lysis hydrochloride assay in human coagulation factor Ⅷ by pre-column derivation[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 362-364,384. doi: 10.3969/j.issn.1006-0111.2018.04.017
shu

Glycine and lysis hydrochloride assay in human coagulation factor Ⅷ by pre-column derivation

doi: 10.3969/j.issn.1006-0111.2018.04.017

  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China

  • Received Date: 2017-11-22
  • Rev Recd Date: 2018-04-18
  • Objective To establish a method of analysis for glycine and lysis hydrochloride in human coagulation factor Ⅷ by pre-column derivation HPLC. Methods The sample was derivatized with 2,4-dinitrofluorobenzene(DNFB). HPLC was performed on a C18 column with gradient elution. The mobile phase A was water, B was acetonitrile and C was 0.05 mol/L sodium acetate buffer containing 4 mmol/L trimethylamine. The flow rate was 1.0 ml/min and detection wavelength was 360nm. Result There was a linear range for glycine between 2.980 g/L~14.90 g/L (r=0.999 9) and lysis hydrochloride between 1.195 g/L~5.975 g/L (r=0.999 9). The average recovery rate of glycine and lysis hydrochloride was 99.1% and 98.2%. Conclusion The established method is accurate and can be used to determine the contents of glycine and lysis hydrochloride in human coagulation factor Ⅷ.
  • [1] 国家药典委员会. 中华人民共和国药典(三部):2015年版[S]. 北京:中国医药科技出版社,2015:272.
    [2] 孙东坡,胡一桥. 蛋白质冷冻干燥制品中的保护剂及其保护机制[J]. 药学进展,2003,27(4):202-205.
    [3] 朱敖兰,杨洁. 生物制品冻干保护剂及其保护机理的研究进展[J]. 喀什师范学院学报,2007,28(6):46-50.
    [4] 高莉萍,李萍,姚佳佳. 高效液相色谱法测定人凝血因子Ⅷ制品中甘氨酸的含量[J]. 药学实践杂志,2016,34(1):59-61.
    [5] 孙向红,张建华. HPLC法测定盐酸赖氨酸的含量[J]. 齐鲁药事,2010(6):345-346.
    [6] 国家药典委员会. 中华人民共和国药典(四部):2015年版[S]. 北京:中国医药科技出版社,2015:235.
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Glycine and lysis hydrochloride assay in human coagulation factor Ⅷ by pre-column derivation

doi: 10.3969/j.issn.1006-0111.2018.04.017
  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China
  • Received Date: 2017-11-22
  • Rev Recd Date: 2018-04-18

Abstract: Objective To establish a method of analysis for glycine and lysis hydrochloride in human coagulation factor Ⅷ by pre-column derivation HPLC. Methods The sample was derivatized with 2,4-dinitrofluorobenzene(DNFB). HPLC was performed on a C18 column with gradient elution. The mobile phase A was water, B was acetonitrile and C was 0.05 mol/L sodium acetate buffer containing 4 mmol/L trimethylamine. The flow rate was 1.0 ml/min and detection wavelength was 360nm. Result There was a linear range for glycine between 2.980 g/L~14.90 g/L (r=0.999 9) and lysis hydrochloride between 1.195 g/L~5.975 g/L (r=0.999 9). The average recovery rate of glycine and lysis hydrochloride was 99.1% and 98.2%. Conclusion The established method is accurate and can be used to determine the contents of glycine and lysis hydrochloride in human coagulation factor Ⅷ.

WANG Chongqing, MA Ningning, YANG Chao, LIU Shihao, ZHU Ju, ZHENG Canhui. Research progress in the catalytic rearrangement reaction of benzyl phenyl ethers[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 486-489. doi: 10.3969/j.issn.1006-0111.2015.06.002
Citation: YAO Jiajia, YAN Chen. Glycine and lysis hydrochloride assay in human coagulation factor Ⅷ by pre-column derivation[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 362-364,384. doi: 10.3969/j.issn.1006-0111.2018.04.017
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