Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats

LIU Cuicui; XIAO Zhijun; LU Saihua; XU Feng

doi:10.3969/j.issn.1006-0111.2019.02.005

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Objective To explore the impact of chronic unpredicted mild stress (CUMS)induced depression on mRNA and protein expression of liver organic anion transporting polypeptides (Oatps) in rats. Methods Twenty-four male SD rats were divided into depression group and control group.Rats in depression group were exposed CUMS for 8 weeks consecutively.Weight,open-field test,sucrose preference,plasma concentration of noradrenaline and corticosterone were assayed before and after model establishment to evaluate the depression.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were measured by RT-qPCR and Western blotting,respectively. Results In CUMS induced depression group,weight,sucrose consumption,vertical score and horizontal score were decreased while levels of corticosterone and norepinephrine were increased compared with control group.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were reduced compared with control group. Conclusion The mRNA and protein expression of liver Oatps in rats were regulated down by CUMS induced depression.

Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats

1. Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China

2. Shanghai Jiaotong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China

Received Date: 2018-06-11

Rev Recd Date: 2018-10-15

Key words:

Abstract: Objective To explore the impact of chronic unpredicted mild stress (CUMS)induced depression on mRNA and protein expression of liver organic anion transporting polypeptides (Oatps) in rats. Methods Twenty-four male SD rats were divided into depression group and control group.Rats in depression group were exposed CUMS for 8 weeks consecutively.Weight,open-field test,sucrose preference,plasma concentration of noradrenaline and corticosterone were assayed before and after model establishment to evaluate the depression.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were measured by RT-qPCR and Western blotting,respectively. Results In CUMS induced depression group,weight,sucrose consumption,vertical score and horizontal score were decreased while levels of corticosterone and norepinephrine were increased compared with control group.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were reduced compared with control group. Conclusion The mRNA and protein expression of liver Oatps in rats were regulated down by CUMS induced depression.

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Reference (13)

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Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats

doi: 10.3969/j.issn.1006-0111.2019.02.005

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