Study on the secondary metabolites of Aspergillus flavipes isolated from coral

WANG Hongliang; JIANG Wenli; LI Ran; XU Congcong; ZHANG Wen

doi:10.3969/j.issn.1006-0111.2019.02.010

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Objective To investigate the active secondary metabolites of coral derived fungi Aspergillus flavipes. Methods Compounds were isolated and purified by means of various chromatographic techniques,including Silica gel column chromatography,Sephadex LH-20 chromatography and HPLC.The structure of the compounds was identified by NMR combined with the data reported.The nuclear factor kappa B receptor activating factor ligand (RANKL) was used to induce bone marrow macrophage cells (BMMs) differentiate into mature osteoclasts.The tartrate-resistant acid phosphatase (TRAP) specific staining was used to test the inhibitory activity of compounds on osteoclast differentiation. Results Four cytochalasins were isolated from the fungus and their structures were identified as trichalasins H,aspergilluchalasin,aspochalasin I and aspochalasin D.Compounds 3 and 4 showed inhibitory activity on osteoclast differentiation. Conclusion This was the first report of inhibiting osteoclast differentiation activity of compounds 3 and 4.These two compounds might have great significance in the study of new anti-osteoporosis drugs.

Study on the secondary metabolites of Aspergillus flavipes isolated from coral

1. Research Center for Marine Drugs, School of Pharmacy, Naval Medical University, Shanghai 200433, China;Sergeant School of Army Medical University, Shijiazhuang 050000, China

2. Research Center for Marine Drugs, School of Pharmacy, Naval Medical University, Shanghai 200433, China

3. Anhui University of Chinese Medicine, Hefei 230038, China

Received Date: 2018-11-04

Rev Recd Date: 2019-03-04

Key words:

Abstract: Objective To investigate the active secondary metabolites of coral derived fungi Aspergillus flavipes. Methods Compounds were isolated and purified by means of various chromatographic techniques,including Silica gel column chromatography,Sephadex LH-20 chromatography and HPLC.The structure of the compounds was identified by NMR combined with the data reported.The nuclear factor kappa B receptor activating factor ligand (RANKL) was used to induce bone marrow macrophage cells (BMMs) differentiate into mature osteoclasts.The tartrate-resistant acid phosphatase (TRAP) specific staining was used to test the inhibitory activity of compounds on osteoclast differentiation. Results Four cytochalasins were isolated from the fungus and their structures were identified as trichalasins H,aspergilluchalasin,aspochalasin I and aspochalasin D.Compounds 3 and 4 showed inhibitory activity on osteoclast differentiation. Conclusion This was the first report of inhibiting osteoclast differentiation activity of compounds 3 and 4.These two compounds might have great significance in the study of new anti-osteoporosis drugs.

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Reference (20)

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Study on the secondary metabolites of Aspergillus flavipes isolated from coral

doi: 10.3969/j.issn.1006-0111.2019.02.010

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