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HUANG Fengai, ZHANG Junping. Research progress on active ingredients from traditional Chinese medicine as inhibitors of PD-1/PD-L1 of cancer immune checkpoint[J]. Journal of Pharmaceutical Practice and Service, 2023, 41(5): 277-283, 290. doi: 10.12206/j.issn.2097-2024.202208079
Citation: WANG Zhijun, YANG Yunyun, ZHANG Wenjing, WANG Xuebin, SONG Hongjie, WANG Zhuo. Determination of voriconazole and its metabolites in human plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 162-165. doi: 10.3969/j.issn.1006-0111.2019.02.012

Determination of voriconazole and its metabolites in human plasma by HPLC

doi: 10.3969/j.issn.1006-0111.2019.02.012
  • Received Date: 2018-08-03
  • Rev Recd Date: 2018-11-30
  • Objective To establish a HPLC method for the determination of the voriconazole concentration in human plasma,which can be used for voriconazole monitoring clinically. Methods High performance liquid chromatography (HPLC-UV) detection method was used with papaverine as internal standard.Acetonitrile protein precipitation method was employed to treat plasma samples.The chromatographic column was ACE5C18-AR150 mm×4.6 mm with the mobile phase 0.025 mol/L sodium dihydrogen phosphate (containing triethylamine 400 μl/L,pH=7.0 adjusted by 0.25 mol/L sodium hydroxide) - acetonitrile (67∶33).The flow rate was l ml/min,the column temperature at 40 ℃,the detection wavelength at 255,276 nm (double wavelength detection) and sampling amount 20 μl. Results The retention time of voriconazole nitrogen oxide,voriconazole and papaverine were 4.5,11.3 and 13.7 min respectively.The linear ranges of voriconazole and voriconazole nitrogen oxides in plasma were 0.5-20.0 μg/ml (r=0.999 5).The quantitative lower limits were 0.5 μg/ml with RSD< 11%.The recovery rates of extraction were between 90.3% and 109.9%. Conclusion This simple and accurate method is suitable for clinical monitoring of voriconazole.
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    [8] BRESSÁN I G,MENDEZ M L,GIMENEZ M I.Validation of a reversed-phase ultra-high-performance liquid chromatographic method with photodiode array detection for the determination of voriconazole in human serum and its application to therapeutic drug monitoring[J].Ther Drug Monit,2018,40(2):276-283.
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    [11] 刘学松,何为群,陈文英,等.肺部真菌感染患者伏立康唑血药浓度监测的临床应用[J].国际呼吸杂志,2016,36(20):1521-1526.
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Determination of voriconazole and its metabolites in human plasma by HPLC

doi: 10.3969/j.issn.1006-0111.2019.02.012

Abstract: Objective To establish a HPLC method for the determination of the voriconazole concentration in human plasma,which can be used for voriconazole monitoring clinically. Methods High performance liquid chromatography (HPLC-UV) detection method was used with papaverine as internal standard.Acetonitrile protein precipitation method was employed to treat plasma samples.The chromatographic column was ACE5C18-AR150 mm×4.6 mm with the mobile phase 0.025 mol/L sodium dihydrogen phosphate (containing triethylamine 400 μl/L,pH=7.0 adjusted by 0.25 mol/L sodium hydroxide) - acetonitrile (67∶33).The flow rate was l ml/min,the column temperature at 40 ℃,the detection wavelength at 255,276 nm (double wavelength detection) and sampling amount 20 μl. Results The retention time of voriconazole nitrogen oxide,voriconazole and papaverine were 4.5,11.3 and 13.7 min respectively.The linear ranges of voriconazole and voriconazole nitrogen oxides in plasma were 0.5-20.0 μg/ml (r=0.999 5).The quantitative lower limits were 0.5 μg/ml with RSD< 11%.The recovery rates of extraction were between 90.3% and 109.9%. Conclusion This simple and accurate method is suitable for clinical monitoring of voriconazole.

HUANG Fengai, ZHANG Junping. Research progress on active ingredients from traditional Chinese medicine as inhibitors of PD-1/PD-L1 of cancer immune checkpoint[J]. Journal of Pharmaceutical Practice and Service, 2023, 41(5): 277-283, 290. doi: 10.12206/j.issn.2097-2024.202208079
Citation: WANG Zhijun, YANG Yunyun, ZHANG Wenjing, WANG Xuebin, SONG Hongjie, WANG Zhuo. Determination of voriconazole and its metabolites in human plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 162-165. doi: 10.3969/j.issn.1006-0111.2019.02.012
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