Rapid identification of chemical constituents of Fuzheng Huayu capsules by UHPLC-TOF/MS

TAO Chaoyang; XING Xinrui; CHEN Si; CAO Qi; DING Deying; ZHU Zhenyu

doi:10.3969/j.issn.1006-0111.2019.05.005

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Objective To identify the chemical constituents in Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time of flight mass spectrometry(UHPLC-TOF/MS). Methods ACQUITY UPLCHSS T3(2.1mm×100mm,1.8μm) was used to chromatographic separation; mobile phase 0.1% formic acid aqueous solution(A) -0.1% formic acid acetonitrile solution(B),gradient elution conditions:0-3min,2% B; 3-18 min,2%-50% B; 18-22 min,50%-95% B; 22-25 min,95% B.The equilibration time was 10 min,the flow rate was 0.40 ml/min,and the analysis time was 25 min.Mass spectrometry was performed by an electrospray ion source using a positive-negative ion mode scan with a range of 100-1 100 m/z. Results 154 components were identified at one time,of which 28 were positive and negative ion modes. Conclusion The composition of Fuzheng Huayu capsule was comprehensively clarified in this study,which laid a foundation for further research of this compound.

Rapid identification of chemical constituents of Fuzheng Huayu capsules by UHPLC-TOF/MS

1. Research Center of Pharmaceutical Preparation, Tibet Military General Hospital, Lhasa 8500771, China

2. Department of Pharmacy, Naval Medical University, Shanghai 200433, China

3. No. 210 Hospital of PLA, Dalian 116000, China

Received Date: 2019-01-09

Rev Recd Date: 2019-03-29

Key words:

Abstract: Objective To identify the chemical constituents in Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time of flight mass spectrometry(UHPLC-TOF/MS). Methods ACQUITY UPLCHSS T3(2.1mm×100mm,1.8μm) was used to chromatographic separation; mobile phase 0.1% formic acid aqueous solution(A) -0.1% formic acid acetonitrile solution(B),gradient elution conditions:0-3min,2% B; 3-18 min,2%-50% B; 18-22 min,50%-95% B; 22-25 min,95% B.The equilibration time was 10 min,the flow rate was 0.40 ml/min,and the analysis time was 25 min.Mass spectrometry was performed by an electrospray ion source using a positive-negative ion mode scan with a range of 100-1 100 m/z. Results 154 components were identified at one time,of which 28 were positive and negative ion modes. Conclusion The composition of Fuzheng Huayu capsule was comprehensively clarified in this study,which laid a foundation for further research of this compound.

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Reference (16)

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Rapid identification of chemical constituents of Fuzheng Huayu capsules by UHPLC-TOF/MS

doi: 10.3969/j.issn.1006-0111.2019.05.005

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