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DING Huamin, GUO Yuchen, QIN Chunxia, SONG Zhibing, SUN Lili. Therapeutic effect of Xiaofeng Zhiyang granules on acute itching in mice with atopic dermatitis by decreasing leukotriene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 211-216. doi: 10.12206/j.issn.2097-2024.202306031
Citation: ZHOU Jing, WU Gao, MA Fujia. Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009

Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p

doi: 10.3969/j.issn.1006-0111.2019.05.009
  • Received Date: 2018-12-11
  • Rev Recd Date: 2019-04-03
  • Objective To study the regulation and mechanism of hsa-miRNA-30a-5p on the proliferation of human lung cancer A549 cells. Methods Five pairs of lung cancer and para-carcinoma tissues were harvested in clinical and measured for hsa-miRNA-30a-5p and SCARA5 levels by real-time PCR and western blotting,respectively.The theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR was predicted by bioinformatics,and validated by luciferase report assay.A549 cells were transfected with pshRNA-SCARA5 and miRNA-30a-5p inhibitor and 48 h later,the proliferation of A549 cells after genetic intervention was assayed by the MTT method,and the cell growth curve was plotted to observe the effect of hsa-miRNA-30a-5p knockdown on cell proliferation. Results For all five pairs of samples tested,hsa-miRNA-30a-5p was higher in the cancer tissues than in the adjacent tissue(P<0.05),and SCARA5 protein was lower in the cancer tissues than in the adjacent tissue (P<0.05).The bioinformatics analysis showed that there was a theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR.The luciferase assay showed that miRNA-30a mimics inhibited the luciferase expressed by the luciferase reporter vector carrying a wild-type SCARA5's 3'UTR(pGL3-WT- SCARA5)(P<0.05,vs pGL3-WT-SCARA5 alone),and miRNA-30a-5p inhibitor enhanced the luciferase activity(P<0.05,vs pGL3-WT- SCARA5 alone).No change was observed when miRNA-30a-5p mimics or miRNA-30a-5p inhibitor was co-transfected with the luciferase reporter vector carrying a mutant SCARA5's 3'UTR(pGL3-MT- SCARA5)(P>0.05,vs pGL3-WT- SCARA5 alone).Hsa-miRNA-30a-5p level in A549 cells transfected with miRNA-30a inhibitor for 48 h was significantly decreased(P<0.05,vs cell control or NC),and there was no difference in hsa-miRNA-30a-5p between the negative control and the transfection control(P>0.05).The proliferation of A549 cells was suppressed by transfection with miRNA-30a-5p inhibitor 24-72 h after transfection(P<0.05,vs control or NC). Conclusion Hsa-miRNA-30a-5p could increase the proliferation in A549 cells via suppressing the expression of SCARA5,and transfection of miRNA-30a-5p inhibitor could inhibit the proliferation of A549 cells via up-regulating SCARA5 expression.
  • [1] 陈万青,张思维,邹小农.中国肺癌发病死亡的估计和流行趋势研究[J].中国肺癌杂志,2010,13(5):488-493.
    [2] 李媛秋,代敏,陈元立,等.中国省区水平肺癌死亡率估计方法研究[J].中国肺癌杂志,2011,14(2):120-126.
    [3] BARTEL D P.MicroRNAs:genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
    [4] ISSAC B,GALOIAN K,GUETTOUCHE T,et al.Genome-wide mRNA and miRNA expression data analysis to screen for markers involved in sarcomagenesis in human chondrosarcoma cell lines[J].Genom Data,2014,2:320-324.
    [5] MISHRA S,YADAV T,RANI V.Exploring miRNA based approaches in cancer diagnostics and therapeutics[J].Crit Rev Oncol Hematol,2016,98:12-23.
    [6] WINTHER M,ALSNER J,TRAMM T,et al.Evaluation of miR-21 and miR-375 as prognostic biomarkers in esophageal cancer[J].Acta Oncol,2015,54(9):1582-1591.
    [7] WANG P,GUO X Y,ZONG W,et al.MicroRNA-128b suppresses tumor growth and promotes apoptosis by targeting A2bR in gastric cancer[J].Biochem Biophys Res Commun,2015,467(4):798-804.
    [8] EGELAND N G,LUNDE S R,JONSDOTTIR K,et al.The role of microRNAs as predictors of response to tamoxifen treatment in breast cancer patients[J].Int J Mol Sci,2015,16(10):24243-24275.
    [9] LI L P,GUO Y,CHEN Y Z,et al.The diagnostic efficacy and biological effects of microRNA-29b for colon cancer[J].Technol Cancer Res Treat,2016,15(6):772-779.
    [10] KNUDSEN K N,NIELSEN B S,LINDEBJERG J,et al.MicroRNA-17 is the most up-regulated member of the mir-17-92 cluster during early colon cancer evolution[J].PLoS One,2015,10(10):e0140503.
    [11] I H,CHO J Y.Lung cancer biomarkers[J].Adv Clin Chem,2015,72:107-170.
    [12] CHEN Z Z,WU Y,MENG Q T,et al.Elevated microRNA-25 inhibits cell apoptosis in lung cancer by targeting RGS3[J].In Vitro Cell Dev Biol Anim,2016,52(1):62-67.
    [13] DONG W,YAO C P,TENG X P,et al.MiR-140-3p suppressed cell growth and invasion by downregulating the expression of ATP8A1 in non-small cell lung cancer[J].Tumour Biol,2016,37(3):2973-2985.
    [14] SONG L,LI D,ZHAO Y K,et al.MiR-218 suppressed the growth of lung carcinoma by reducing MEF2D expression[J].Tumour Biol,2016,37(3):2891-2900.
    [15] LIN L,LIN H B,WANG L,et al.MiR-130a regulates macrophage polarization and is associated with non-small cell lung cancer[J].Oncol Rep,2015,34(6):3088-3096.
    [16] SONG Y F,HONG J F,LIU D L,et al.MiR-630 targets LMO3 to regulate cell growth and metastasis in lung cancer[J].Am J Transl Res,2015,7(7):1271-1279.
    [17] MA R Q,WANG C Y,WANG J J,et al.MiRNA-mRNA interaction network in non-small cell lung cancer[J].Interdiscip Sci,2016,8(3):209-219.
    [18] HUANG J,ZHENG D L,QIN F S,et al.Genetic and epigenetic silencing of SCARA5 may contribute to human hepatocellular carcinoma by activating FAK signaling[J].J Clin Invest,2010,120(1):223-241.
    [19] KHAMAS A,ISHIKAWA T,SHIMOKAWA K,et al.Screening for epigenetically masked genes in colorectal cancer Using 5-Aza-2'-deoxycytidine,microarray and gene expression profile[J].Cancer Genom Proteomics,2012,9(2):67-75.
    [20] YAN N,ZHANG S,YANG Y,et al.Therapeutic upregulation of class A scavenger receptor member 5 inhibits tumor growth and metastasis[J].Cancer Sci,2012,103(9):1631-1639.
    [21] LIU J,HU G,CHEN D,et al.Suppression of SCARA5 by Snail1 is essential for EMT-associated cell migration of A549 cells[J].Oncogenesis,2013,2:e73.
    [22] WEI W,YANG Y,CAI J,et al.MiR-30a-5p suppresses tumor metastasis of human colorectal cancer by targeting ITGB3[J].Cell Physiol Biochem,2016,39(3):1165-1176.
    [23] BARANISKIN A,BIRKENKAMP-DEMTRODER K,MAGHNOUJ A,et al.MiR-30a-5p suppresses tumor growth in colon carcinoma by targeting DTL[J].Carcinogenesis,2012,33(4):732-739.
    [24] FRANZETTI G A,LAUD-DUVAL K,BELLANGER D,et al.MiR-30a-5p connects EWS-FLI1 and CD99,two major therapeutic targets in Ewing tumor[J].Oncogene,2013,32(33):3915-3921.
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Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p

doi: 10.3969/j.issn.1006-0111.2019.05.009

Abstract: Objective To study the regulation and mechanism of hsa-miRNA-30a-5p on the proliferation of human lung cancer A549 cells. Methods Five pairs of lung cancer and para-carcinoma tissues were harvested in clinical and measured for hsa-miRNA-30a-5p and SCARA5 levels by real-time PCR and western blotting,respectively.The theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR was predicted by bioinformatics,and validated by luciferase report assay.A549 cells were transfected with pshRNA-SCARA5 and miRNA-30a-5p inhibitor and 48 h later,the proliferation of A549 cells after genetic intervention was assayed by the MTT method,and the cell growth curve was plotted to observe the effect of hsa-miRNA-30a-5p knockdown on cell proliferation. Results For all five pairs of samples tested,hsa-miRNA-30a-5p was higher in the cancer tissues than in the adjacent tissue(P<0.05),and SCARA5 protein was lower in the cancer tissues than in the adjacent tissue (P<0.05).The bioinformatics analysis showed that there was a theoretical binding site of hsa-miRNA-30a-5p in SCARA5's 3'UTR.The luciferase assay showed that miRNA-30a mimics inhibited the luciferase expressed by the luciferase reporter vector carrying a wild-type SCARA5's 3'UTR(pGL3-WT- SCARA5)(P<0.05,vs pGL3-WT-SCARA5 alone),and miRNA-30a-5p inhibitor enhanced the luciferase activity(P<0.05,vs pGL3-WT- SCARA5 alone).No change was observed when miRNA-30a-5p mimics or miRNA-30a-5p inhibitor was co-transfected with the luciferase reporter vector carrying a mutant SCARA5's 3'UTR(pGL3-MT- SCARA5)(P>0.05,vs pGL3-WT- SCARA5 alone).Hsa-miRNA-30a-5p level in A549 cells transfected with miRNA-30a inhibitor for 48 h was significantly decreased(P<0.05,vs cell control or NC),and there was no difference in hsa-miRNA-30a-5p between the negative control and the transfection control(P>0.05).The proliferation of A549 cells was suppressed by transfection with miRNA-30a-5p inhibitor 24-72 h after transfection(P<0.05,vs control or NC). Conclusion Hsa-miRNA-30a-5p could increase the proliferation in A549 cells via suppressing the expression of SCARA5,and transfection of miRNA-30a-5p inhibitor could inhibit the proliferation of A549 cells via up-regulating SCARA5 expression.

DING Huamin, GUO Yuchen, QIN Chunxia, SONG Zhibing, SUN Lili. Therapeutic effect of Xiaofeng Zhiyang granules on acute itching in mice with atopic dermatitis by decreasing leukotriene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 211-216. doi: 10.12206/j.issn.2097-2024.202306031
Citation: ZHOU Jing, WU Gao, MA Fujia. Study on the mechanism of proliferation of human lung cancer A549 cells regulated by hsa-miRNA-30a-5p[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 433-439. doi: 10.3969/j.issn.1006-0111.2019.05.009
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