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ZENG Lingjun, KE Xiaowen, SONG Hongtao. Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009
Citation:
HAN Ling, WANG Xinxia, LV Lei, ZHANG Jingru, KAN Yueyi, LU Ying, FU Xiang. Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007
Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC
Department of Pharmacy, Shanghai Kongjiang Hospital, Shanghai 200093, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China
2.
Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
3.
School of Pharmacy, Naval Medical University, Shanghai 200433, China
4.
Department of Pharmacy, Shanghai Kongjiang Hospital, Shanghai 200093, China
Received Date: 2019-08-20
Rev Recd Date:
2019-11-04
Abstract
Objective To establish a HPLC method for the simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream. Methods The HPLC condition was as follows.The column was Agilent Extend-C18 (4.6 mm×150 mm,5 μm).The mobile phase was methanol and ammonium acetate buffer solution (20 mmol/L,pH=7.5).Gradient elution.The methanol ratio varies with time as follows:0-10 min,methanol→60%; 10-12 min,methanol 60%→92%;12-20 min,methanol→92%.Acquisition time was 20 minutes.Liquid phase equilibrium time was 10 min.Flow rate was 1 ml/min.UV detection wavelength was 218 nm.Column temperature was 25℃.Injection volume was 15 μl. Results The three components were separated at baseline within 30 min.The methodological verification showed that the standard curves had good linearity (r>0.9995).The inter-day precision were less than 3.0%.The recovery was between 90% and 108%.The RSD% of stability and repeatability were also less than 3.0%.The results showed that the method could meet the requirements of method validation.The contents of the three components in three batches were determined according to the newly established method. Conclusion The method is simple and reliable,can provide a basis for the quality control of multi-effect protective cream,and laid a foundation for its quality standard research.
Abstract: Objective To establish a HPLC method for the simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream. Methods The HPLC condition was as follows.The column was Agilent Extend-C18 (4.6 mm×150 mm,5 μm).The mobile phase was methanol and ammonium acetate buffer solution (20 mmol/L,pH=7.5).Gradient elution.The methanol ratio varies with time as follows:0-10 min,methanol→60%; 10-12 min,methanol 60%→92%;12-20 min,methanol→92%.Acquisition time was 20 minutes.Liquid phase equilibrium time was 10 min.Flow rate was 1 ml/min.UV detection wavelength was 218 nm.Column temperature was 25℃.Injection volume was 15 μl. Results The three components were separated at baseline within 30 min.The methodological verification showed that the standard curves had good linearity (r>0.9995).The inter-day precision were less than 3.0%.The recovery was between 90% and 108%.The RSD% of stability and repeatability were also less than 3.0%.The results showed that the method could meet the requirements of method validation.The contents of the three components in three batches were determined according to the newly established method. Conclusion The method is simple and reliable,can provide a basis for the quality control of multi-effect protective cream,and laid a foundation for its quality standard research.
ZENG Lingjun, KE Xiaowen, SONG Hongtao. Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009
Citation:
HAN Ling, WANG Xinxia, LV Lei, ZHANG Jingru, KAN Yueyi, LU Ying, FU Xiang. Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007
ZENG Lingjun, KE Xiaowen, SONG Hongtao. Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009
ZENG Lingjun, KE Xiaowen, SONG Hongtao. Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009
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