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Volume 38 Issue 1
Mar.  2020
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2020  >  38(1): 74-76

SHENG Fengxian, ZHANG Youjie. Determination of glycine in human thrombin by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 74-76. doi: 10.3969/j.issn.1006-0111.201903048
Citation: SHENG Fengxian, ZHANG Youjie. Determination of glycine in human thrombin by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 74-76. doi: 10.3969/j.issn.1006-0111.201903048
shu

Determination of glycine in human thrombin by HPLC

doi: 10.3969/j.issn.1006-0111.201903048

  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China

  • Received Date: 2019-03-12
  • Rev Recd Date: 2019-05-13
  • Objective To establish a HPLC method for glycine assay in the human thrombin. Methods The sample was derivatized with 2,4-dinitrofluorobenzene (DNFB). HPLC was performed on an ODS-C18 column with 50% acetonitrile –0.05mol/L sodium acetate buffer (35:65) as the mobile phase. The flow rate was 1.0 ml/min and detection wavelength at 360 nm. Results The linear range of glycine was 0.006-0.030 mg/ml (r=0.999 6). The average recovery was 100.4%, with RSD 0.44% (n=9). Conclusion This method is simple, accurate and specific. It is suitable for glycine assay in human thrombin.
  • [1] 薛毅, 王庆才, 徐俊, 等. 纤维蛋白胶对实验性肝损伤的止血效果观察[J]. 中华实验外科杂志, 1996, 13(3):151-152
    [2] 薛毅, 王庆才. 纤维蛋白胶在实验性皮肤损伤愈合中的作用[J]. 中华实验外科杂志, 1997, 14(4):255
    [3] 孙东坡, 胡一桥. 蛋白质冷冻干燥制品中的保护剂及其保护机制[J]. 药学进展, 2003, 27(4):201-205
    [4] 朱敖兰, 杨洁, ZHU A L, 等. 生物制品冻干保护剂及其保护机理的研究进展[J]. 喀什师范学院学报, 2007, 28(6):46-50
    [5] 国家药典委员会. 中华人民共和国药典(四部)2015年版[S]. 北京: 中国医药科技出版社, 2015: 235-236.
    [6] 丁建, 王玲娜, 苏丹. RP-HPLC法测定眼氨肽滴眼液中6种氨基酸的含量[J]. 江苏药学与临床研究, 2004, 12(5):29-32
    [7] 高莉萍, 李萍, 姚佳佳, 等. 高效液相色谱法测定人凝血因子Ⅷ制品中甘氨酸的含量[J]. 药学实践杂志, 2016, 34(1):59-61
    [8] 姚佳佳, 闫晨. 柱前衍生化法同时测定人凝血因子Ⅷ中甘氨酸和盐酸赖氨酸的含量[J]. 药学实践杂志, 2018, 36(4):362-364
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Determination of glycine in human thrombin by HPLC

doi: 10.3969/j.issn.1006-0111.201903048
  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China
  • Received Date: 2019-03-12
  • Rev Recd Date: 2019-05-13

Abstract: Objective To establish a HPLC method for glycine assay in the human thrombin. Methods The sample was derivatized with 2,4-dinitrofluorobenzene (DNFB). HPLC was performed on an ODS-C18 column with 50% acetonitrile –0.05mol/L sodium acetate buffer (35:65) as the mobile phase. The flow rate was 1.0 ml/min and detection wavelength at 360 nm. Results The linear range of glycine was 0.006-0.030 mg/ml (r=0.999 6). The average recovery was 100.4%, with RSD 0.44% (n=9). Conclusion This method is simple, accurate and specific. It is suitable for glycine assay in human thrombin.

SHENG Fengxian, ZHANG Youjie. Determination of glycine in human thrombin by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 74-76. doi: 10.3969/j.issn.1006-0111.201903048
Citation: SHENG Fengxian, ZHANG Youjie. Determination of glycine in human thrombin by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 74-76. doi: 10.3969/j.issn.1006-0111.201903048
shu

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