Content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis spectrophotometry

LI Jie; YANG Yuru; WANG Qingfen; ZHENG Shaozhong; CHEN Jinshan

doi:10.3969/j.issn.1006-0111.201904019

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Objective To establish a method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis Spectrophotometry. Methods The contents of total flavones and polysaccharides in Fangshu Qingre mixture were determined by UV-Vis spectroscopy with rutin and anhydrous glucose as reference substance, and the wavelength was set at 508 nm and 487 nm. Results The contents were from 0.00 to 59.20 μg/ml for total flavones and from 10.92 to 109.20 μg/ml for total polysaccharides in Fangshu Qingre mixture. The recoveries of total flavones and total polysaccharides were 104.4% and 104.8% respectively. Conclusion The method of using ultraviolet spectroscopy was simple, reproducible, accurate and reliable, which could be preferably used as the method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture.

Content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis spectrophotometry

Department of Pharmacy, 909 th Hospital of Joint Service Support Force/Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China

Received Date: 2019-04-03

Rev Recd Date: 2019-10-22

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Abstract: Objective To establish a method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis Spectrophotometry. Methods The contents of total flavones and polysaccharides in Fangshu Qingre mixture were determined by UV-Vis spectroscopy with rutin and anhydrous glucose as reference substance, and the wavelength was set at 508 nm and 487 nm. Results The contents were from 0.00 to 59.20 μg/ml for total flavones and from 10.92 to 109.20 μg/ml for total polysaccharides in Fangshu Qingre mixture. The recoveries of total flavones and total polysaccharides were 104.4% and 104.8% respectively. Conclusion The method of using ultraviolet spectroscopy was simple, reproducible, accurate and reliable, which could be preferably used as the method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture.

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Content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis spectrophotometry

doi: 10.3969/j.issn.1006-0111.201904019

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