Determination of puerarin in Gegen qinlian pian by RP-HPLC
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Key words:
- RP-HPLC /
- Gegen Qinglian Pian /
- Puerarin
Abstract: A quantitative method was developed for the determination of puerarin in Gegen Qing lian Pian by reversed phase HPLC.Chromatographic conditions included column ODS-C(10),column temperature,35℃;detector UV,250nm;mobile phase,methanol-water(23:77),flow rate 1 ml/min;injecting volume,10μl;external standad.the number of theoretical plates calculated by puerarin peak was no less than 2000.The standard curve was linear in the concentration range of 5μg/ml-80μg/ml,and correlation coefficient was 0.9999.The average rccovery and the relative standsrd deviation were 97.6% and 1.8%,respectively.
Citation: | Tan Shengjian, Qiu Xia, Li Guanghui, Zhao Bingcheng, Liang Yuqing. Determination of puerarin in Gegen qinlian pian by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1996, (5): 295-297. |