HPLC determination of caffeine metabolites in urine

Yi Tao; Lu Jianfen

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Volume 15 Issue 2

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Article Navigation > Journal of Pharmaceutical Practice and Service > 1997 > (2): 94-99

Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.

Citation:

Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.

HPLC determination of caffeine metabolites in urine

Yi Tao¹,
Lu Jianfen²

1.
Wuhan General Hospital of PLA Wuhan 430070
2.

Abstract

A HPLC method was developed for determination of caffeine metabolites in urine(AFMU, 1X, 1U, 17X, 17U).The samplb added ammonium sulfate was extracted twice by a mixture of chloroform-isopropanol(85:15 vol/vol)and evaporated to dryness with a gentle stream ofair below)25℃.HPLC analysis was carried out on Shim Pack C₁₈ column with methanol-ace-tonitrile-0.05% acetic acid (10:1:89 vol/vol) as the mobile phase and UV detection at 280nm.The assay procedure has been applied to determining urinary metabolites excreted after oral caffeine in a healthy sample(n = 120).Caffeine metabolite ratios were used to assess the poymorphicN-acetyltransferase and CYP₁A₂ activities.
- Caffeine,
- HPLC,
- N-acetyltransferase,
- CYP₁A₂
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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