Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.
Citation: Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.

HPLC determination of caffeine metabolites in urine

  • A HPLC method was developed for determination of caffeine metabolites in urine(AFMU, 1X, 1U, 17X, 17U).The samplb added ammonium sulfate was extracted twice by a mixture of chloroform-isopropanol(85:15 vol/vol)and evaporated to dryness with a gentle stream ofair below)25℃.HPLC analysis was carried out on Shim Pack C18 column with methanol-ace-tonitrile-0.05% acetic acid (10:1:89 vol/vol) as the mobile phase and UV detection at 280nm.The assay procedure has been applied to determining urinary metabolites excreted after oral caffeine in a healthy sample(n = 120).Caffeine metabolite ratios were used to assess the poymorphicN-acetyltransferase and CYP1A2 activities.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(3510) PDF downloads(442) Cited by()

Related
Proportional views

HPLC determination of caffeine metabolites in urine

Abstract: A HPLC method was developed for determination of caffeine metabolites in urine(AFMU, 1X, 1U, 17X, 17U).The samplb added ammonium sulfate was extracted twice by a mixture of chloroform-isopropanol(85:15 vol/vol)and evaporated to dryness with a gentle stream ofair below)25℃.HPLC analysis was carried out on Shim Pack C18 column with methanol-ace-tonitrile-0.05% acetic acid (10:1:89 vol/vol) as the mobile phase and UV detection at 280nm.The assay procedure has been applied to determining urinary metabolites excreted after oral caffeine in a healthy sample(n = 120).Caffeine metabolite ratios were used to assess the poymorphicN-acetyltransferase and CYP1A2 activities.

Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.
Citation: Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return