Determination of amlodipine in serum by HPLC
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Key words:
- amlodipine /
- HPLC /
- the determination of conten
Abstract: A rapid specific and sensitive HPLC menthod for the determination of amlodipine in serum was developed, using a stainless steel column (ods-5s, 150×4.0mm) with nifedipine as internal standard. The mobile phase was a mixture of methanol-KH2PO4 buffer(0.03mol/L), flow rate 1ml/min. Wavelength of the detector was set at 300nm. The linear correlattion was observed within the range of 4-12μg/ml(r=0.9992) and detect limitation at 4ng. The mean recoveries of the method were 100.24±5.4O% (intra-day)and 99.87±6.23% (inter-day).
Citation: | Xi Ronggang, Wang Xiaobo, Xing Shanmin, Jin Zhujie. Determination of amlodipine in serum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 1998, (3): 135-137. |