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Volume 18 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2000  >  (4): 225-227

CHEN Sai-zhen, YUAN Guo-ping, HU Da-ping, CHEN Yue-bin. Determination of rifampicin concentration in human serum by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2000, (4): 225-227.
Citation: CHEN Sai-zhen, YUAN Guo-ping, HU Da-ping, CHEN Yue-bin. Determination of rifampicin concentration in human serum by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2000, (4): 225-227.
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Determination of rifampicin concentration in human serum by RP-HPLC

  • Received Date: 2000-02-20
  • OBJECTIVE To determine the concentration of rifampicin in human serum by RP-HPLC.METHODS Serum was precipitated with centrifugation after add ed 4 times of methanol,and its super-stratum clear fluid was injected after filtration with 0.45μ micro-pore filter membrane.The method used Nova-Pak C18 column as chromatogram column,methanol-acetate buffer solution as flow phase,chlordiazepoxide as internal standard.The detection wavelength was 334nm.RESULTS The linear range of rifampicin was 1～25μg/ml.Average recovery was (100.9±4.2)%.Daily and daytime RSD were less than 4%.The lowest detection limit was 0.5μg/ml(the rate of signal and noise was greater than or equal to 3).CONCLUSION This method is simple,reliable and indicated for clinical monitoring.
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Determination of rifampicin concentration in human serum by RP-HPLC

  • Received Date: 2000-02-20

Abstract: OBJECTIVE To determine the concentration of rifampicin in human serum by RP-HPLC.METHODS Serum was precipitated with centrifugation after add ed 4 times of methanol,and its super-stratum clear fluid was injected after filtration with 0.45μ micro-pore filter membrane.The method used Nova-Pak C18 column as chromatogram column,methanol-acetate buffer solution as flow phase,chlordiazepoxide as internal standard.The detection wavelength was 334nm.RESULTS The linear range of rifampicin was 1～25μg/ml.Average recovery was (100.9±4.2)%.Daily and daytime RSD were less than 4%.The lowest detection limit was 0.5μg/ml(the rate of signal and noise was greater than or equal to 3).CONCLUSION This method is simple,reliable and indicated for clinical monitoring.

CHEN Sai-zhen, YUAN Guo-ping, HU Da-ping, CHEN Yue-bin. Determination of rifampicin concentration in human serum by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2000, (4): 225-227.
Citation: CHEN Sai-zhen, YUAN Guo-ping, HU Da-ping, CHEN Yue-bin. Determination of rifampicin concentration in human serum by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2000, (4): 225-227.
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