Attenuates pulmonary dysfunction induced by ischaemia-reperfusion of lower extremitates in rats

Key words: 
• G-CSF /
• ischaemia-reperfusion /
• pulmonary dysfunction

Abstract: OBJECTIVE To investigate the therapeutic efficacy of Granulocyte colony-stimulating factor (G-CSF) against rat pulmonary dysfunction induced by ischaemia-reperfusion of lower extremities.METHODS Male Spague-Dawley rats were randomised into three group.The operated and G-CSF group underwent laparotomy and clamping of the infrarenal abdominal aorta for 120min followed by 120min of reperfusion; The sham-operated group underwent laparot-my for 240min only;The G-CSF group was pretreated with G-CSF 20μ_g/kg through iv and the other given 1ml saline each.A Ⅱ groups reeived Evan's blue dye 30mg/kg iv, preoperatively. Concentrations of lung malonyldiaclehyde (MDA) and Evan's blue blye were measured as marker of lung injury. RESZJLTS Lung MDA level of G-CSF group was(1.71±0.34)nmol/mg;Sham-operated group was(1.73±0.65)nmol/mg,both significantly lower than that of operated group(2.54±0.39)nmol/mg. Even's blue dye concentration was significantly higher in operated group(3.07±1.18)μ_g/mg than in G-CSF group(1.50±0.29)μ_g/mg and sham-operated group(0.13±0.07)μ_g/mg. CONCLUSIGN G-CSF has therapeutic efficacy against pulmonary dysfunction which was induced by ischaemia-reperfusion of lower extremitates in rats.