Detemination of rutaecarpine In Zuojin Wan by HPLC

Key words: 
• HPLC /
• Zuojin wan /
• rutaecarpine

Abstract: OBJECTIVE A quantitative method was developed for the determination of rutaecarpine in Zuojin Wan by HPLC. METHOD The chromatographic conditions include column Intersil C₁₈, mobile phase:acetonitrile-10% acetonitrile (50:50), flow rate 1 ml·min^-1, wavelength 345nm. RESULTS The number of theoretical plates caculated by rutaecarpine peak was 2100, The regression equation of rutaecarpine was Y=-0.2197+0.000000349X(r=0.9999) and the liner range was 12-60μg·ml^-1. The average recovery of rutaecarpine was 96.9% (n=5),RSD 3.7%. The measurable lowest limit of rutaecarpine was 0.1μg·ml^-. CONCLUSION The method is sensitive, rapid and accurate.