Determination of baicalin in Qinlingzi vagina perfusate by RP-HPLC
- Received Date: 2002-03-06
-
Key words:
- baicalin /
- Qinlingzi vagina perfusate /
- RP-HPLC.
Abstract: OBJECTIVE To establish a reversed-phase HPLC method for determination of contents of baicalin in Qinlingzi vagina perfusate.METHODS RP-HPLC analysis was carried out by using spheridorb C18 column and methanol water-phosphoie(51:49:0.1) as the mobile the detection wavelength was 278nm. The concentratinn of baiealin was quantified by external standard. RESULTS The method was linear with the range of 5.0-80.0μg·ml-1,the regression equation was A=32365.56C-1997.75, r=0.9999(n=5),the average recovery was 97.85%(n=5) and the RSD was 0.86%(n=6).CONCLUSION The method is convenient, rapid and reproducible, it may be used for the quantitative determination of baicalin in Qinlingzi vagina perfusate.
Citation: | SONG Hong-ying, LIU Fang. Determination of baicalin in Qinlingzi vagina perfusate by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 286-288. |