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Volume 20 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2002  >  (5): 286-288

WANG Bin, HUANG He-zhou, ZHU Zhen-yu, ZHANG Hai, JI Song-gang, CHAI Yi-feng. Blending of crude Epimedium Brevicornum according to their HPLC fingerprints[J]. Journal of Pharmaceutical Practice and Service, 2008, (6): 432-434,456.
Citation: SONG Hong-ying, LIU Fang. Determination of baicalin in Qinlingzi vagina perfusate by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 286-288.
shu

Determination of baicalin in Qinlingzi vagina perfusate by RP-HPLC

  • 1.

    The Pharmaceutical Factory of Zhejiang Academy of Medical Sciences Hangzhou 310013, China

  • 2.

    The Institute of Materia Medica of Zhejiang Academy of Medical Sciences Hangzhou 310013, China

  • Received Date: 2002-03-06
  • OBJECTIVE To establish a reversed-phase HPLC method for determination of contents of baicalin in Qinlingzi vagina perfusate.METHODS RP-HPLC analysis was carried out by using spheridorb C18 column and methanol water-phosphoie(51:49:0.1) as the mobile the detection wavelength was 278nm. The concentratinn of baiealin was quantified by external standard. RESULTS The method was linear with the range of 5.0-80.0μg·ml-1,the regression equation was A=32365.56C-1997.75, r=0.9999(n=5),the average recovery was 97.85%(n=5) and the RSD was 0.86%(n=6).CONCLUSION The method is convenient, rapid and reproducible, it may be used for the quantitative determination of baicalin in Qinlingzi vagina perfusate.
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Determination of baicalin in Qinlingzi vagina perfusate by RP-HPLC

  • 1. The Pharmaceutical Factory of Zhejiang Academy of Medical Sciences Hangzhou 310013, China
  • 2. The Institute of Materia Medica of Zhejiang Academy of Medical Sciences Hangzhou 310013, China
  • Received Date: 2002-03-06

Abstract: OBJECTIVE To establish a reversed-phase HPLC method for determination of contents of baicalin in Qinlingzi vagina perfusate.METHODS RP-HPLC analysis was carried out by using spheridorb C18 column and methanol water-phosphoie(51:49:0.1) as the mobile the detection wavelength was 278nm. The concentratinn of baiealin was quantified by external standard. RESULTS The method was linear with the range of 5.0-80.0μg·ml-1,the regression equation was A=32365.56C-1997.75, r=0.9999(n=5),the average recovery was 97.85%(n=5) and the RSD was 0.86%(n=6).CONCLUSION The method is convenient, rapid and reproducible, it may be used for the quantitative determination of baicalin in Qinlingzi vagina perfusate.

WANG Bin, HUANG He-zhou, ZHU Zhen-yu, ZHANG Hai, JI Song-gang, CHAI Yi-feng. Blending of crude Epimedium Brevicornum according to their HPLC fingerprints[J]. Journal of Pharmaceutical Practice and Service, 2008, (6): 432-434,456.
Citation: SONG Hong-ying, LIU Fang. Determination of baicalin in Qinlingzi vagina perfusate by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 286-288.
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