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LIN Pie-ying, WU Xiang-feng, LIU Li-li, XING Wang-xing. Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 289-290.
Citation: LIN Pie-ying, WU Xiang-feng, LIU Li-li, XING Wang-xing. Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 289-290.

Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis

  • Received Date: 2002-04-02
  • OBJECTIVE To determine the contents of diethylstilbestrol by capillary zone electroporesis(CZE).METHODS Using cinnamon acid as the internal standard(IS),the electrophoretic separation was achieved with 25 mmol·L-1borate(pH=9.18) as the running buffer and a voltage of 24 kV was applied to the 45cm×50μm(i.d) capillary.The analysis were introduced into capillary by siphon(1s) and determined with on-column monitoring at 214nm. RESULTS The determination could be completed w ithin 4 min and the minimum concentration of detection was 10μg·ml-1.The analytical results of diethylstilbestrol samples demonstraoted a good linear relationship within the 105.7-528.5μg·ml-1.The relative standard deviation (RSD)of intra-day was less than 1.61% and that of inter-day was less 3.76%.The present recoveries were bettween 97.91%-101.58%.CONCLUSION This method is accurate, simple, sensitive and reliable, which can be applied to the determination of diethylstilbestrol content.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis

Abstract: OBJECTIVE To determine the contents of diethylstilbestrol by capillary zone electroporesis(CZE).METHODS Using cinnamon acid as the internal standard(IS),the electrophoretic separation was achieved with 25 mmol·L-1borate(pH=9.18) as the running buffer and a voltage of 24 kV was applied to the 45cm×50μm(i.d) capillary.The analysis were introduced into capillary by siphon(1s) and determined with on-column monitoring at 214nm. RESULTS The determination could be completed w ithin 4 min and the minimum concentration of detection was 10μg·ml-1.The analytical results of diethylstilbestrol samples demonstraoted a good linear relationship within the 105.7-528.5μg·ml-1.The relative standard deviation (RSD)of intra-day was less than 1.61% and that of inter-day was less 3.76%.The present recoveries were bettween 97.91%-101.58%.CONCLUSION This method is accurate, simple, sensitive and reliable, which can be applied to the determination of diethylstilbestrol content.

LIN Pie-ying, WU Xiang-feng, LIU Li-li, XING Wang-xing. Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 289-290.
Citation: LIN Pie-ying, WU Xiang-feng, LIU Li-li, XING Wang-xing. Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 289-290.

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