Studies on the determination of indigotin and indirubin contents in Indigo Naturalis with Supercritical Fluid Extraction
- Received Date: 2002-06-10
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Key words:
- indigotin /
- indirubin /
- UV spectrophotometry /
- first order derivative UV spectrophotometry /
- contents determination
Abstract: OBJECTIVE To determine the contents of indigotin and indirubin in Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction. METHODS The indirubin content was determined with sulfonate reaction-first order derivative-UV spectrophotometry; the indigotin content was determined with sulfonated reaotion-UV spectrophotometry.RESULTS The average recovery rate of indirubin was 101.80%,RSD=0.75%,and the average recovery rate of indigotin was 101.06%,RSD=1.27%.CONCLUSTION This method is simple and repeatable, and can be used as the quality control for method for the Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction.
Citation: | YAO Wen, WANG Xiao-bo, XI Rong-gang. Studies on the determination of indigotin and indirubin contents in Indigo Naturalis with Supercritical Fluid Extraction[J]. Journal of Pharmaceutical Practice and Service, 2002, (5): 291-293. |