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Volume 22 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2004  >  (4): 228-230

ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.
Citation: ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.
shu

Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography

  • Institute for Drug and Instrument Control of Shenyang Military Region, Shenyang 110026, China

  • Received Date: 2004-01-01
  • Objective To develop a method for determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography. Methods ODS C18 (4.6mm×250mm, 7μm) was selected and the mobile phase consisted of acetonitrile -0.4% phosphoric acid (15:85)at the flow rate 1mL/min. The detection wavelength was 327nm. ResultsThe calibration curve was linear in the range of 1.62-21.60μg(r =1.0000). The average recovery of the method was 100.1%,RSD was 0.80% (n = 5). Conclusion This method was reliable and accurate. The method can be used to control the quality of preparation.
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Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography

  • Institute for Drug and Instrument Control of Shenyang Military Region, Shenyang 110026, China
  • Received Date: 2004-01-01

Abstract: Objective To develop a method for determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography. Methods ODS C18 (4.6mm×250mm, 7μm) was selected and the mobile phase consisted of acetonitrile -0.4% phosphoric acid (15:85)at the flow rate 1mL/min. The detection wavelength was 327nm. ResultsThe calibration curve was linear in the range of 1.62-21.60μg(r =1.0000). The average recovery of the method was 100.1%,RSD was 0.80% (n = 5). Conclusion This method was reliable and accurate. The method can be used to control the quality of preparation.

ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.
Citation: ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.
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