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ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.
Citation: ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.

Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography

  • Received Date: 2004-01-01
  • Objective To develop a method for determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography. Methods ODS C18 (4.6mm×250mm, 7μm) was selected and the mobile phase consisted of acetonitrile -0.4% phosphoric acid (15:85)at the flow rate 1mL/min. The detection wavelength was 327nm. ResultsThe calibration curve was linear in the range of 1.62-21.60μg(r =1.0000). The average recovery of the method was 100.1%,RSD was 0.80% (n = 5). Conclusion This method was reliable and accurate. The method can be used to control the quality of preparation.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography

Abstract: Objective To develop a method for determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography. Methods ODS C18 (4.6mm×250mm, 7μm) was selected and the mobile phase consisted of acetonitrile -0.4% phosphoric acid (15:85)at the flow rate 1mL/min. The detection wavelength was 327nm. ResultsThe calibration curve was linear in the range of 1.62-21.60μg(r =1.0000). The average recovery of the method was 100.1%,RSD was 0.80% (n = 5). Conclusion This method was reliable and accurate. The method can be used to control the quality of preparation.

ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.
Citation: ZHANG Guang-chun, ZHAO Xin, WANG Xu-ming, CHEN Jun. Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 228-230.

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