Determination of atropine sulfate eye drops by ion-pair HPLC
- Received Date: 2004-03-04
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Key words:
- atropine sulfate /
- eye drops /
- ion-pair HPLC /
- determination
Abstract: Objective An ion-pair HPLC method was developed for determination of atropine sulfate in atropine sulfate eye drops. Methods Alltech-C18 (4.6×250mm, 5μm)column was used and the mobile phase was acetonitrile -0.05mol/L sodium hep-tanesul fonate (35:65). It was detected at 210nm. ResultsLinearity was obtained in the concentration rage of 8-120μg/mL, A=-72915.4073 + 15308.1639C,r=0.999. The average recoveries was 100.2%, RSD = 0.65%. Conclusion The method provide a accurate and sensitive way for the determination of atropine sulfate in atropine sulfate eye drops by ion-pair HPLC.
Citation: | LI Zhi-mei, LIU Meng, ZHANG Huang-dan. Determination of atropine sulfate eye drops by ion-pair HPLC[J]. Journal of Pharmaceutical Practice and Service, 2004, (4): 230-232. |