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WU Ji, SUN Yan, WANG Yun-chuan, JIAO Ling, ZHANG Li. Determination of endotxin in three intravenous mixed injection by kinetic turbidimetric assay[J]. Journal of Pharmaceutical Practice and Service, 2005, (1): 18-20.
Citation: WU Ji, SUN Yan, WANG Yun-chuan, JIAO Ling, ZHANG Li. Determination of endotxin in three intravenous mixed injection by kinetic turbidimetric assay[J]. Journal of Pharmaceutical Practice and Service, 2005, (1): 18-20.

Determination of endotxin in three intravenous mixed injection by kinetic turbidimetric assay

  • Received Date: 2003-07-22
  • Objective To approach the feasibility of replacing pyrogen test with bacterial endotoxin test (BET) by analysing the interference of balance salts containing glucose, tinidazole and glucose, ofloxacin and glucose injection to limulus amebocyte lysate (LAL).Methods The kinetic turbidimetric assay (KTA) was used to determinate the bacterial endotoxin and did an interfering test by using of the EDS-99 bacterial endoxin systems, in the same time gel-clot method was used to test and verify.Results The experiment shows the balance salts containing glucose, tinidazole and glucose injection have no interference to LAL, ofloxacin and glucose injection has no interference to LAL when is diluted four times.Conclusion The gel-clot method can be used as a daily examination to analyse balance salts containing glucose, tinidazole and glucose,ofloxacin and glucose injection.
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Determination of endotxin in three intravenous mixed injection by kinetic turbidimetric assay

Abstract: Objective To approach the feasibility of replacing pyrogen test with bacterial endotoxin test (BET) by analysing the interference of balance salts containing glucose, tinidazole and glucose, ofloxacin and glucose injection to limulus amebocyte lysate (LAL).Methods The kinetic turbidimetric assay (KTA) was used to determinate the bacterial endotoxin and did an interfering test by using of the EDS-99 bacterial endoxin systems, in the same time gel-clot method was used to test and verify.Results The experiment shows the balance salts containing glucose, tinidazole and glucose injection have no interference to LAL, ofloxacin and glucose injection has no interference to LAL when is diluted four times.Conclusion The gel-clot method can be used as a daily examination to analyse balance salts containing glucose, tinidazole and glucose,ofloxacin and glucose injection.

WU Ji, SUN Yan, WANG Yun-chuan, JIAO Ling, ZHANG Li. Determination of endotxin in three intravenous mixed injection by kinetic turbidimetric assay[J]. Journal of Pharmaceutical Practice and Service, 2005, (1): 18-20.
Citation: WU Ji, SUN Yan, WANG Yun-chuan, JIAO Ling, ZHANG Li. Determination of endotxin in three intravenous mixed injection by kinetic turbidimetric assay[J]. Journal of Pharmaceutical Practice and Service, 2005, (1): 18-20.

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