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Volume 23 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2005  >  (2): 99-102

ZHOU Yong-gang, HUANG Sheng, GU Li, WANG Bin, ZHANG Guo-qing, LOU Zi-yang. Determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (2): 99-102.
Citation: ZHOU Yong-gang, HUANG Sheng, GU Li, WANG Bin, ZHANG Guo-qing, LOU Zi-yang. Determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (2): 99-102.
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Determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae by HPLC

  • Objective To establish the method for determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae. Method The content was determined by HPLC. A Zorbax Eclipse XDB-C18 column(4.6mm×250mm,5μm) with a grient mobile phase composed of 25 mmol/L dihydrophosphate potassium and acetonitrile were used. The detection wavelength was 257 nm and the flow rate was 1.0mL/min. Results The calibration curves of mangiferin were linear between 14.2-568.5μg/mL(r=0.9999), the neo-mangiferin were 14.8-590.5μg/mL. The withinday precision RSD was both less than 4.5% and the interday precision RSD was less than 3.9%. Conclusion The method is simple, rapid, accurate and is suitable for the determination of mangiferin and neo-mangiferin.
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Determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae by HPLC

Abstract: Objective To establish the method for determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae. Method The content was determined by HPLC. A Zorbax Eclipse XDB-C18 column(4.6mm×250mm,5μm) with a grient mobile phase composed of 25 mmol/L dihydrophosphate potassium and acetonitrile were used. The detection wavelength was 257 nm and the flow rate was 1.0mL/min. Results The calibration curves of mangiferin were linear between 14.2-568.5μg/mL(r=0.9999), the neo-mangiferin were 14.8-590.5μg/mL. The withinday precision RSD was both less than 4.5% and the interday precision RSD was less than 3.9%. Conclusion The method is simple, rapid, accurate and is suitable for the determination of mangiferin and neo-mangiferin.

ZHOU Yong-gang, HUANG Sheng, GU Li, WANG Bin, ZHANG Guo-qing, LOU Zi-yang. Determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (2): 99-102.
Citation: ZHOU Yong-gang, HUANG Sheng, GU Li, WANG Bin, ZHANG Guo-qing, LOU Zi-yang. Determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (2): 99-102.
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