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Volume 23 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2005  >  (3): 163-165

ZHENG Yi-min, XU Xiu-ying, YANG Yan-hong, FU Shan-quan. Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (3): 163-165.
Citation: ZHENG Yi-min, XU Xiu-ying, YANG Yan-hong, FU Shan-quan. Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (3): 163-165.
shu

Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC

  • College of Bioengineer, Chongqing Institue of Technology, Chongqing 400050, China

  • Received Date: 2004-09-01
  • Objective To determine the content of hyperoside and oleanolic acid in Diospyros kaki.Methods The HPLC method was used to determine the content of hyperoside and oleanolic acid in Diospyros kaki.The analysis of hyperoside was carried out on Shi madzu C18column (150mm×4.6mm,5μm).The mobile phase was MeOH-0.2%H3PO4(45:55).The flow-rate was 1.0mL/min.The detection wave-length was 350nm.The analysis of oleanolic acid was carried out on ShimadzuC18column(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3PO4(90:10).The flow -rate was 0.8 mL/min.The detection wave -length was 220nm.Results The liner range of hyperoside was 0.50-2.50μg and gave a correlation (r) of 0.9999.The recovery was 99.65% and RSD was 1.24 % .The liner range of oleanolic acid was1.56-5.20μg and gave a correlation (r) of 0.9990.The recovery was 97.02% and RSD was 2.84 % .Conclusion The method is simple, fast and had a good linear relationship.It is to determine the content of hyperoside and oleanolic acid in Diospyros kaki.
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Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC

  • College of Bioengineer, Chongqing Institue of Technology, Chongqing 400050, China
  • Received Date: 2004-09-01

Abstract: Objective To determine the content of hyperoside and oleanolic acid in Diospyros kaki.Methods The HPLC method was used to determine the content of hyperoside and oleanolic acid in Diospyros kaki.The analysis of hyperoside was carried out on Shi madzu C18column (150mm×4.6mm,5μm).The mobile phase was MeOH-0.2%H3PO4(45:55).The flow-rate was 1.0mL/min.The detection wave-length was 350nm.The analysis of oleanolic acid was carried out on ShimadzuC18column(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3PO4(90:10).The flow -rate was 0.8 mL/min.The detection wave -length was 220nm.Results The liner range of hyperoside was 0.50-2.50μg and gave a correlation (r) of 0.9999.The recovery was 99.65% and RSD was 1.24 % .The liner range of oleanolic acid was1.56-5.20μg and gave a correlation (r) of 0.9990.The recovery was 97.02% and RSD was 2.84 % .Conclusion The method is simple, fast and had a good linear relationship.It is to determine the content of hyperoside and oleanolic acid in Diospyros kaki.

ZHENG Yi-min, XU Xiu-ying, YANG Yan-hong, FU Shan-quan. Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (3): 163-165.
Citation: ZHENG Yi-min, XU Xiu-ying, YANG Yan-hong, FU Shan-quan. Determination of hyperoside and oleanolic acid in Diospyros kaki by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (3): 163-165.
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