Determination of palonosetron metabolism in human hepatic microsome by RP HPLC
- Received Date: 2005-01-11
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Key words:
- palonosetron /
- RP-HPLC /
- metabolism /
- microsomes
Abstract: Objective To establish a RP-HPLC method for determinate palonosetron in human hepatic microsomes.Methods Pal onosetron in human hepatic microsomal incubates was assayed on a Nova-park C18(5μm, 200×4.6mm) column with a mobile phase of methanol-0.01mol/L KH2PO4 (80:20, v/v) at a flow-rate of 1.0 mL/min.A UV-VIS detector was operated at 240nm.Results The assay was linear from 5-100 μmol/L for palonosetron (r= 0.999 8 ).The limit of detection was 0.2 μmol/L ( signal to noise ratio≥3) and the limit of quantification was 1.0μmol/L (RSD =4.88%,n=3).The method afforded recoveries of 96.0%-103.0% (n=5), and inter-day and intra-day variation coefficient were <7% and 10% (n=5).Conclusion The method is simple, accurate and can be used to study the metabolism of palonosetron in human hepatic microsomes.
Citation: | CHEN Chang-shui, YANG Xian-qi. Determination of palonosetron metabolism in human hepatic microsome by RP HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (3): 169-171. |