Determination of baicalin in Zhenhuang Wan by RP-HPLC

HU Si-yi; XU Xiao-wei

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Volume 23 Issue 4

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Article Navigation > Journal of Pharmaceutical Practice and Service > 2005 > (4): @-@

HU Si-yi, XU Xiao-wei. Determination of baicalin in Zhenhuang Wan by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (4): @-@.

Citation:

HU Si-yi, XU Xiao-wei. Determination of baicalin in Zhenhuang Wan by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2005, (4): @-@.

Determination of baicalin in Zhenhuang Wan by RP-HPLC

The First Affiliated Hospital of Wenzhou Medical College of Zhejiang Province, Wenzhou 325000, China

Received Date: 2004-12-06

Abstract

Objective To develop a RP-HPLC method for determination of baicalin in Zhenhuang Wan.Methods Alltima C18 column was used and methanol-water-acetic acid glacial (50 : 50 :1) was used as mobile phase,detection wavelegth was 273nm.Results There was a good linear relationship between the concentration of baicalin and absorption area value in range of 7.444 - 37.220μg r = 0.999 7.The average recovery was 98.55% with RSD=0.65% (n=5).Conclusion The method was repeatable and accurate.
- RP-HPLC,
- Zhen huang Wan,
- baicalin
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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